Determination of the chelatable iron pool of isolated rat hepatocytes by digital fluorescence microscopy using the fluorescent probe, phen green SK.
AuthorsPetrat F, Rauen U, de Groot H
JournalHepatology
PubMed ID10094962
'The intracellular pool of chelatable iron is considered to be a decisive pathogenetic factor for various kinds of cell injury. We therefore set about establishing a method of detecting chelatable iron in isolated hepatocytes based on digital fluorescence microscopy. The fluorescence of hepatocytes loaded with the fluorescent metal indicators, phen ... More
Luminescent CdS quantum dots as selective ion probes.
AuthorsChen Y, Rosenzweig Z
JournalAnal Chem
PubMed ID12380840
'Water-soluble luminescent CdS quantum dots (QDs) capped by polyphosphate, L-cysteine, and thioglycerol were synthesized in aqueous solution. The ligands were found to have a profound effect on the luminesence response of CdS QDs to physiologically important metal cations. Polyphosphate-capped CdS QDs were sensitive to nearly all mono- and divalent cations, ... More
Binding of zinc(II) and copper(II) to the full-length Alzheimer's amyloid-beta peptide.
AuthorsTõugu V, Karafin A, Palumaa P,
JournalJ Neurochem
PubMed ID18289347
'There is evidence that binding of metal ions like Zn2+ and Cu2+ to amyloid beta-peptides (Abeta) may contribute to the pathogenesis of Alzheimer''s disease. Cu2+ and Zn2+ form complexes with Abeta peptides in vitro; however, the published metal-binding affinities of Abeta vary in an enormously large range. We studied the ... More
Copper transport by lobster (Homarus americanus) hepatopancreatic mitochondria.
AuthorsChavez-Crooker P, Garrido N, Ahearn GA
JournalJ Exp Biol
PubMed ID11854377
Mechanisms of copper transport into purified mitochondrial suspensions prepared from the hepatopancreas of the Atlantic lobster Homarus americanus were investigated. Mitochondria were purified by combining methods of differential and Percoll-gradient centrifugation, and copper transport was studied using the copper-sensitive fluorescent dye Phen Green. Copper transport by this mitochondrial preparation was ... More
Membrane ion channels as physiological targets for local Ca2+ signalling.
AuthorsGordienko DV, Zholos AV, Bolton TB
JournalJ Microsc
PubMed ID10594771
Ionized calcium plays a central role as a second messenger in a number of physiologically important processes determining smooth muscle function. To regulate a wide range of cellular activities the mechanisms of subcellular calcium signalling should be very diverse. Recent progress in development of visible light-excitable fluorescent dyes with high ... More
Copper transport by lobster hepatopancreatic epithelial cells separated by centrifugal elutriation: measurements with the fluorescent dye Phen Green.
AuthorsChavez-Crooker P, Garrido N, Ahearn GA
JournalJ Exp Biol
PubMed ID11273805
The hepatopancreas of the American lobster (Homarus americanus) possesses four types of epithelial cells arranged along blind-ended tubules. At the distal tips of these tubules, stem cells termed E-cells differentiate into three other cell types, R-cells, F-cells and B-cells, each of which have different absorptive and secretory roles in the ... More