Differential translocation of protein kinase C epsilon during HeLa cell adhesion to a gelatin substratum.
AuthorsChun JS, Ha MJ, Jacobson BS
JournalJ Biol Chem
PubMed ID8662811
'The spreading of HeLa cells, following attachment to a collagen or gelatin substratum, requires the activation of protein kinase C (PKC). Membrane-bound PKC was previously shown to be activated during cell attachment and in response to the activation of a series of lipid second messengers turned on by the ligation ... More
Localized measurement of kinase activation in oocytes of Xenopus laevis.
'We have combined a rapid cytoplasmic sampling technique with capillary electrophoresis to measure the activation of protein kinase C (PKC) in a small region (approximately 60 microm) of a Xenopus oocyte. The phosphorylation of a fluorescent PKC substrate was measured following addition of a pharmacological or physiological stimulus to an ... More
Inositol trisphosphate receptor: phosphorylation by protein kinase C and calcium calmodulin-dependent protein kinases in reconstituted lipid vesicles.
AuthorsFerris CD, Huganir RL, Bredt DS, Cameron AM, Snyder SH
JournalProc Natl Acad Sci U S A
PubMed ID1848697
'We have previously demonstrated that the inositol 1,4,5-trisphosphate (IP3) receptor is phosphorylated by cyclic AMP-dependent protein kinase (PKA). In the present study, phosphorylation of IP3 receptors has been examined with purified receptor protein reconstituted in liposomes to remove detergent that can inhibit protein kinases. The IP3 receptor is stoichiometrically phosphorylated ... More