'Mobility of taxol inside microtubules was investigated using fluorescence recovery after photobleaching on flow-aligned bundles. Bundles were made of microtubules with either GMPCPP or GTP at the exchangeable site on the tubulin dimer. Recovery times were sensitive to bundle thickness and packing, indicating that taxol molecules are able to move ... More
Myosin-II inhibition and soft 2D matrix maximize multinucleation and cellular projections typical of platelet-producing megakaryocytes.
AuthorsShin JW, Swift J, Spinler KR, Discher DE,
JournalProc Natl Acad Sci U S A
PubMed ID21709232
'Cell division, membrane rigidity, and strong adhesion to a rigid matrix are all promoted by myosin-II, and so multinucleated cells with distended membranes--typical of megakaryocytes (MKs)--seem predictable for low myosin activity in cells on soft matrices. Paradoxically, myosin mutations lead to defects in MKs and platelets. Here, reversible inhibition of ... More
Use of fluorochrome-tagged taxol to produce fluorescent microtubules in solution.
AuthorsMelan MA
JournalBiotechniques
PubMed ID9714874
Accumulation and distribution of doxorubicin in tumour spheroids: the influence of acidity and expression of P-glycoprotein.
AuthorsMellor HR, Callaghan R,
JournalCancer Chemother Pharmacol
PubMed ID21400240
The intra-tumour distribution of anticancer drugs remains an important, but often under-estimated, influence on drug efficacy. Tumour acidity and the presence of efflux pumps were examined for their influence on the distribution of doxorubicin in a solid tumour model. Anticancer drug distribution and overall accumulation was measured in tumour spheroids ... More