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Abstract
The location of fluorescence probes with charged groups in model membranes.
AuthorsKachel K, Asuncion-Punzalan E, London E
JournalBiochim Biophys Acta
PubMed ID9814853
'The location of commonly used charged fluorescent membrane probes in membranes was determined in order to: (1) investigate the relationship between the structure of hydrophobic molecules and their depth within membranes; and (2) aid interpretation of experiments in which these fluorescent probes are used to examine membrane structure. Membrane depth ... More
Nondestructive detection of neutral glycosphingolipids with lipophilic anionic fluorochromes and their employment for preparative high-performance thin-layer chromatography.
AuthorsMüthing J, Kemminer SE
JournalAnal Biochem
PubMed ID8660611
'In this study a simple and effective procedure for the isolation of individual neutral glycosphingolipids (GSLs) by preparative thin-layer chromatography is described. The method is based on nondestructive visualization of neutral GSLs on silica gel precoated thin-layer chromatography plates with anionic lipophilic fluorochromes. After thin-layer chromatography, individual neutral GSLs were ... More
Pyruvate as a fluorescence quencher: a new spectroscopic assay for pyruvate reactions.
AuthorsAndo T, Miyata H
JournalAnal Biochem
PubMed ID6222667
'Pyruvate ion, which is biologically ubiquitous and participates in many metabolic reactions, was found to be an effective quencher of fluorescence. Compared to other negatively charged quenchers such as I-, pyruvate is not toxic to proteins. By adding an inert, long-lived fluorophore to systems transacting pyruvate, it is possible to ... More
Membrane studies with polarity-dependent and excimer-forming fluorescent probes.
'1. The interaction of electron-transporting particles from heavy mitochondria of ox heart with several fluorescent probes was examined. 2. 1-Anilinonaphthalene-8-sulphonate and 2-(N-methylanilino)naphthalene-6-sulphonate both show an energy-dependent response. 3. Energy transfer between the electron-transporting particles and the dyes and the kinetics of the dye-particle interaction were studied in order to locate ... More
Ligand binding and physicochemical characteristics of an IgM mouse plasmacytoma ABPC-22.
AuthorsJarvis MR, Voss EW
JournalMol Immunol
PubMed ID6795452
The study of rous sarcoma virus-transformed baby hamster kidney cells using fluorescent probes.
AuthorsEdwards HE, Thomas JK, Burleson GR, Kulpa CF
JournalBiochim Biophys Acta
PubMed ID184839
The fluorescent probes pyrene, pyrene butyric acid and N-phenyl 1-naphthylamine have been used to investigate the changes that accompany in vitro transformation of a baby hamster kidney cell line using Rous sarcoma virus. The fluorescent probes which reside in the membrane were used to compare the changes in microviscosity ... More
Organization and dynamics of pyrene and pyrene lipids in intact lipid bilayers. Photo-induced charge transfer processes.
AuthorsBarenholz Y, Cohen T, Korenstein R, Ottolenghi M
JournalBiophys J
PubMed ID1883931
The dynamics of fluorescence quenching and the organization of a series of pyrene derivatives anchored in various depths in bilayers of phosphatidylcholine small unilamellar vesicles was studied and compared with their behavior in homogeneous solvent systems. The studies include characterization of the environmental polarity of the pyrene fluorophore based on ... More
Kinetic processes in Escherichia coli membranes and cells. A laser photolysis study using derivatives of pyrene.
AuthorsWong M, Kulpa CF, Thomas JK
JournalBiochim Biophys Acta
PubMed ID769828
Pyrene and several derivatives of pyrene are used to investigate photo-induced kinetic processes in whole cells and membranes extracted from Escherichia coli. A mutant of E. coli was used which, under appropriate growth conditions, produced a complete or incomplete lipopolysaccharide in the outer membrane. The pyrene derivatives used were: pyrene ... More
A search for site-filling ligands in the Mcg Bence-Jones dimer: crystal binding studies of fluorescent compounds.
AuthorsEdmundson AB, Ely KR, Herron JN
JournalMol Immunol
PubMed ID6431268
In trigonal crystals grown in 1.9 M ammonium sulfate buffered at pH 6.2, the Mcg light-chain (Bence-Jones) dimer has a highly aromatic binding cavity accessible to a wide range of hydrophobic and aromatic ligands. A search was made for site-filling ligands by diffusing compounds into the crystals and determining their ... More