Post-column reaction detection based on fluorescence energy transfer in the far red spectral region.
AuthorsShahdeo K,Karnes HT
JournalJournal of pharmaceutical and biomedical analysis
PubMed ID10703992
Simultaneous multicolor fluorescence cross-correlation spectroscopy to detect higher order molecular interactions using single wavelength laser excitation.
AuthorsHwang LC, Gösch M, Lasser T, Wohland T
JournalBiophys J
PubMed ID16632502
'Fluorescence cross-correlation spectroscopy is a powerful method for the study of molecular interactions and dynamics in solution and even in living cells. Usually, in the optical setup, either two laser beams have to be superimposed in their respective confocal volumes or two-photon excitation is used for a dual-color detection system. ... More
P-selectin glycoprotein ligand-1 supports rolling on E- and P-selectin in vivo.
'Selectin-dependent rolling is the earliest observable event in the recruitment of leukocytes to inflamed tissues. Several glycoproteins decorated with sialic acid, fucose, and/or sulfate have been shown to bind the selectins. The best-characterized selectin ligand is P-selectin glycoprotein-1 (PSGL-1) that supports P-selectin- dependent rolling in vitro and in vivo. In ... More
The effect of IL-2 treatment on transcriptional attenuation in proto-oncogenes pim-1 and c-myb in human thymic blast cells.
AuthorsRohwer F, Todd S, McGuire KL
JournalJ Immunol
PubMed ID8752912
IL-2 is the major mitogenic cytokine for mature human T cells. This growth factor has been shown previously to induce the expression of a number of genes, including structural proteins, proto-oncogenes, and metabolic enzymes. Multiple mechanisms, including increases in mRNA stability, protein synthesis, and new transcriptional initiation, have been studied ... More
A TAT-streptavidin fusion protein directs uptake of biotinylated cargo into mammalian cells.
AuthorsAlbarran B, To R, Stayton PS
JournalProtein Eng Des Sel
PubMed ID15820981
The HIV-1 TAT peptide has been used extensively for directing the intracellular delivery of an assortment of cargo, including DNA, liposomes and macromolecules. For protein delivery, a variety of TAT-fusion proteins have been described which link the TAT coding sequence to the protein coding sequence of interest. Streptavidin represents a ... More