Functional protein microarrays: ripe for discovery.
AuthorsPredki PF,
JournalCurr Opin Chem Biol
PubMed ID15036150
'The manufacture and use of protein microarrays with correctly folded and functional content presents significant challenges. Despite this, the feasibility and utility of such undertakings are now clear, and exciting progress has recently been demonstrated in the areas of content generation, printing strategies and protein immobilization. More importantly, we are ... More
Development of functional protein microarrays for drug discovery: progress and challenges.
AuthorsZhou FX, Bonin J, Predki PF,
JournalComb Chem High Throughput Screen
PubMed ID15379625
'Functional protein microarrays promise new approaches to address longstanding challenges in drug discovery and development, with applications ranging from target identification to clinical trial design. However, their widespread adoption will be contingent upon a robust ability to develop and manufacture arrays in support of these applications. This review will address ... More
A Network-based Analysis of Polyanion-binding Proteins Utilizing Yeast Protein Arrays.
AuthorsSalamat-Miller N, Fang J, Seidel CW, Smalter AM, Assenov Y, Albrecht M, Middaugh CR,
JournalMol Cell Proteomics
PubMed ID16982674
'The high affinity of certain cellular polyanions for many proteins (polyanion-binding proteins (PABPs)) has been demonstrated previously. It has been hypothesized that such polyanions may be involved in protein structure stabilization, stimulation of folding through chaperone-like activity, and intra- and extracellular protein transport as well as intracellular organization. The purpose ... More
Global analysis of protein phosphorylation in yeast.
AuthorsPtacek J, Devgan G, Michaud G, Zhu H, Zhu X, Fasolo J, Guo H, Jona G, Breitkreutz A, Sopko R, McCartney RR, Schmidt MC, Rachidi N, Lee SJ, Mah AS, Meng L, Stark MJ, Stern DF, De Virgilio C, Tyers M, Andrews B, Gerstein M, Schweitzer B, Predki PF, Snyder M,
JournalNature
PubMed ID16319894
Protein phosphorylation is estimated to affect 30% of the proteome and is a major regulatory mechanism that controls many basic cellular processes. Until recently, our biochemical understanding of protein phosphorylation on a global scale has been extremely limited; only one half of the yeast kinases have known in vivo substrates ... More