Invitrogen

KDEL Polyclonal Antibody

Name: KDEL Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-013
Price: 2188.00 CNY
Availability: InStock

高级验证

12个已发表图片

27 篇参考文献

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说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

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KDEL Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 18

KDEL Antibody (PA1-013) in ICC/IF

Immunofluorescent analysis of KDEL in U251 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a KDEL polyclonal antibody (Product # PA1-013) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552). KDEL staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

KDEL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

KDEL Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)

KDEL Antibody in Immunohistochemistry (IHC)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

KDEL Antibody — FIGURE 5. Differential VCAM-1 production between murine airway smooth muscle and epithelial cells in response to poly(I,C). Airway smooth muscle and epithelial cells were treated without ( NT ; lanes 1 and 4 ) or with poly(I,C) ( PIC ; lanes 2 and 5 ) or tunicamycin ( TUN ; lanes 3 and 6 ). These blots were probed with antibodies against KDEL ( A, green ) and VCAM-1 ( B, green ). Molecular weight ladders are shown in red / yellow . beta-Actin ( red ) is shown for each lane as a loading control. The KDEL and VCAM bands ( A and B , respectively) were quantified and presented in C and D , respectively. Error bars , S.D. ( n = 3). {TM}

产品信息

PA1-013

应用

建议稀释比

已发表文章

免疫印迹 (WB)

8-16 µg/mL

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看6篇已发表文章 6篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:200

免疫细胞化学 (ICC/IF)

Assay-dependent

查看15篇已发表文章 15篇已发表文章

流式细胞分析 (Flow)

1-20 µg/mL

免疫沉淀 (IP)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Hamster, Human, Mouse, Rat

已发表种属

Hamster, Human, Mouse, Non-human primate, Rat, Sea urchin

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL once reconstituted

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C, Avoid Freeze/Thaw Cycles

Ambient (domestic); Wet ice (international)

AB_325593

产品详细信息

PA1-013 detects KDEL from human, rat, mouse and hamster.

PA1-013 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 57 kDa protein representing protein disulfide isomerase (PDI), a 78 kDa protein representing glucose regulated protein 70 kDa (GRP78) and an ~94 kDa protein representing GRP94 in mouse myeloma cells. Immunofluorescence staining of GRP78 in mouse myeloma cells with PA1-013 results in diffuse cytoplasmic staining consistent with specific ER localization. PA1-013 immunoprecipitates PDI, GRP78, an ~72 kDa protein which may be endoplasmic reticulum protein 72 kDa (ERp72), and an ~94 kDa protein representing GRP94 from numerous rodent tissues.

The PA1-013 immunogen is a synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94.

Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.

靶标信息

The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: ER lumen protein retaining receptor 1; KDEL; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1; KDEL (Lys-Asp-Glu-Leu) receptor; KDELR1; KDEL endoplasmic reticulum protein retention receptor 1; KDEL receptor 1; Lys-Asp-Glu-Leu; putative MAPK-activating protein PM23; RP23-446N16.1; unnamed protein product

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基因别名: 8030486F04Rik; ERD2; ERD2.1; HDEL; I79_020931; PM23

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UniProt ID: (Human) P24390, (Mouse) Q99JH8, (Rat) Q569A6