Search
Invitrogen
Ghrelin Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
图: 1 / 5
Ghrelin Antibody (PA1-1070) in ICC/IF
Immunofluorescence analysis of GHRL was performed using 70% confluent log phase MKN-45 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ghrelin Rabbit Polyclonal Antibody (Product # PA1-1070) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA1-1070
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues F(1) N A P F D V G I K L S G A Q Y Q Q H G R A L(23) of the rat Obestatin gene.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-1070 detects Obestatin in mouse and rat samples. This product does not detect endogenous levels of the hormone in rat stomach.
PA1-1070 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~2.5 kDa protein representing synthetic Obestatin.
The PA1-1070 immunogen is a synthetic peptide corresponding to residues F(1) N A P F D V G I K L S G A Q Y Q Q H G R A L(23) of the rat Obestatin gene. This sequence is conserved in mouse and only one amino acid residue difference in human. This peptide (Cat. # PEP-290) is available for use in neutralization and control experiments.
靶标信息
GHRL encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an the ligand for the growth hormone secretagogue receptor type 1 and is involved in regulating growth hormone release. Ghrelin has an appeptite-stimulating effect and induces adiposity and stimulates gastric acid secretion. Obestatin may be the ligand for the orphan G protein-coupled receptor GPR39 and is involved in satiety and can have an appetite-reducing effect resulting in decreased food intake.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: AMCBX1; Appetite-regulating hormone; FIP-3; FIP3; Fip3p; ghrelin; ghrelin 27; ghrelin 28; ghrelin/obestatin; Growth hormone secretagogue; Growth hormone-releasing peptide; IKK-gamma; IP; IP1; IP2; IPD2; Motilin-related peptide; NEMO; OTTHUMP00000207794; OTTHUMP00000207795; OTTHUMP00000207796; OTTHUMP00000207798; preproghrelin; proghrelin; Protein M46
基因别名: 2210006E23Rik; Ghr; GHRL; m46; MTLRP; MTLRPAP; UNQ524/PRO1066
UniProt ID: (Rat) Q9QYH7, (Mouse) Q9EQX0
Entrez Gene ID: (Rat) 59301, (Mouse) 58991
activation of MAPK activity
gastric acid secretion
negative regulation of endothelial cell proliferation
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
actin polymerization or depolymerization
adult feeding behavior
response to hormone
hormone-mediated signaling pathway
dendrite development
positive regulation of insulin secretion
regulation of response to food
positive regulation of response to food
positive regulation of appetite
negative regulation of interleukin-1 beta production
negative regulation of locomotion
regulation of cell proliferation
negative regulation of circadian sleep/wake cycle, REM sleep
negative regulation of tumor necrosis factor biosynthetic process
negative regulation of apoptotic process
response to estrogen
negative regulation of interleukin-6 biosynthetic process
positive regulation of circadian sleep/wake cycle, non-REM sleep
negative regulation of insulin secretion
decidualization
negative regulation of inflammatory response
positive regulation of corticotropin secretion
positive regulation of cortisol secretion
positive regulation of synapse assembly
excitatory postsynaptic potential
positive regulation of growth hormone secretion
positive regulation of protein tyrosine kinase activity
negative regulation of energy homeostasis
positive regulation of energy homeostasis
visual learning
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
