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Invitrogen

HMGB1 Polyclonal Antibody

Advanced Verification
7 Published Figures
13 References
View all (56) HMGB1 antibodies

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HMGB1 Polyclonal Antibody

Product Details

PA1-16926

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-1 µg/mL
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:250
View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

0.05 µg/mL
View 3 publications 3 publications

Flow Cytometry (Flow)

1:100
-

ELISA (ELISA)

Assay-Dependent
-

ChIP assay (ChIP)

5 µg/mL
-

Functional Assay (Functional)

-
View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Sheep, Rabbit, Rat

Published species

Dog, Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2248274

Product Specific Information

This antibody is expected to cross react with porcine, equine and rabbit samples based on 100% sequence homology.

Suggested positive control: Hela whole cell extract, antigen standard for HMGB1 (transient overexpression lysate).

Target Information

High-mobility group box 1 protein, previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Amphoterin; Amphoterin antibody; DKFZp686A04236; Heparin-binding protein p30; high mobility group 1; High mobility group protein 1; High mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility-group protein; HMG-1; HMG3 antibody; hmgb 1; hmgb-1; HMGB1; RP11-550P23.1; Sulfoglucuronyl carbohydrate binding protein; unnamed protein product

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Gene Aliases: Ac2-008; amphoterin; DEF; HMG-1; HMG1; HMG3; HMGB1; p30; SBP-1

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UniProt ID: (Human) P09429, (Bovine) P10103, (Dog) Q6YKA4, (Rat) P63159, (Mouse) P63158

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Entrez Gene ID: (Human) 3146, (Rabbit) 100328641, (Bovine) 282691, (Sheep) 101112071, (Dog) 403170, (Rat) 25459, (Mouse) 15289

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Function(s)
four-way junction DNA binding bubble DNA binding transcription regulatory region sequence-specific DNA binding lipopolysaccharide binding phosphatidylserine binding DNA binding damaged DNA binding double-stranded DNA binding single-stranded DNA binding transcription coactivator activity transcription corepressor activity RNA binding double-stranded RNA binding single-stranded RNA binding cytokine activity integrin binding protein binding lipid binding DNA binding, bending calcium-dependent protein kinase regulator activity lyase activity C-X-C chemokine binding protein kinase activator activity chemoattractant activity receptor agonist activity RAGE receptor binding RNA polymerase II sequence-specific DNA binding transcription factor binding DNA polymerase binding supercoiled DNA binding DNA-binding transcription factor binding histone binding non-sequence-specific DNA binding, bending unmethylated CpG binding repressing transcription factor binding open form four-way junction DNA binding crossed form four-way junction DNA binding bent DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding 5S rRNA binding transcription factor binding heparin binding peptide binding poly(A) RNA binding protein dimerization activity glycolipid binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter eye development myeloid dendritic cell activation endothelial cell proliferation positive regulation of endothelial cell proliferation activation of innate immune response adaptive immune response plasmacytoid dendritic cell activation macrophage activation involved in immune response myeloid progenitor cell differentiation immune system process dendritic cell chemotaxis inflammatory response to antigenic stimulus regulation of tolerance induction regulation of T cell mediated immune response to tumor cell glycogen catabolic process DNA metabolic process DNA topological change DNA repair base-excision repair double-strand break repair double-strand break repair via nonhomologous end joining DNA recombination chromatin organization chromatin remodeling transcription from RNA polymerase II promoter autophagy chemotaxis inflammatory response immune response cellular response to DNA damage stimulus signal transduction positive regulation of cytosolic calcium ion concentration positive regulation of autophagy negative regulation of endothelial cell migration negative regulation of RNA polymerase II transcriptional preinitiation complex assembly myeloid cell differentiation lung development neuron projection development heterochromatin assembly regulation of restriction endodeoxyribonuclease activity DNA geometric change positive regulation of mismatch repair negative regulation of interferon-gamma production positive regulation of chemokine production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interleukin-1 beta production positive regulation of interleukin-1 production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production V(D)J recombination positive regulation of toll-like receptor 2 signaling pathway positive regulation of toll-like receptor 4 signaling pathway positive regulation of toll-like receptor 9 signaling pathway T-helper 1 cell activation endothelial cell chemotaxis positive regulation of activated T cell proliferation positive regulation of apoptotic process apoptotic cell clearance negative regulation of CD4-positive, alpha-beta T cell differentiation positive regulation of DNA binding positive regulation of MAPK cascade positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration T-helper 1 cell differentiation innate immune response positive regulation of innate immune response positive regulation of cell differentiation positive regulation of myeloid cell differentiation positive regulation of glycogen catabolic process positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of viral entry into host cell cell development regulation of viral process positive chemotaxis regulation of DNA metabolic process response to glucocorticoid positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide positive regulation of monocyte chemotactic protein-1 production positive regulation of monocyte chemotaxis positive regulation of wound healing neutrophil clearance cellular response to interleukin-7 positive regulation of NIK/NF-kappaB signaling positive regulation of sprouting angiogenesis regulation of hemopoiesis positive regulation of myeloid progenitor cell differentiation positive regulation of vascular endothelial cell proliferation positive regulation of chemokine (C-X-C motif) ligand 2 production negative regulation of apoptotic cell clearance regulation of nucleotide-excision repair positive regulation of dendritic cell differentiation cell morphogenesis positive regulation of protein phosphorylation positive regulation of mesenchymal cell proliferation base-excision repair, DNA ligation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter nervous system development circadian rhythm negative regulation of DNA replication positive regulation of cell proliferation response to heat response to glucose positive regulation of cell death positive regulation of neuron projection development positive regulation of smooth muscle cell migration positive regulation of cell migration actin cytoskeleton reorganization response to lipopolysaccharide response to insulin positive regulation of myeloid cell apoptotic process response to interferon-gamma response to drug positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of myoblast differentiation positive regulation of protein kinase activity positive regulation of mitotic cell cycle regulation of inflammatory response male-specific defense response to bacterium induction of positive chemotaxis myoblast proliferation cellular response to interleukin-1 B cell proliferation involved in immune response nucleosome assembly activation of protein kinase activity toll-like receptor 9 signaling pathway DNA ligation involved in DNA repair positive regulation of DNA ligation tumor necrosis factor secretion chromatin silencing
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