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Invitrogen
TDP-43 Polyclonal Antibody
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FIGURE: 1 / 10
TDP-43 Antibody (PA1-16996) in ICC/IF
Immunocytochemistry analysis of TDP-43 in HeLa cells. Samples were incubated with TDP-43 polyclonal antibody (Product # PA1-16996) using a dilution of 2 µg/mL overnight at 4°C followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-16996
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Non-human primate,
Xenopus,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide to a C-terminal region [within residues 350-414] of the human TARDBP protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive control: Hela whole cell extract, antigen standard for TARDBP (transient overexpression lysate).
Target Information
TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3"e; splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: OTTHUMP00000002171; OTTHUMP00000002173; RP4-635E18.2; TAR DNA-binding protein 43; TDP-43
Gene Aliases: 1190002A23Rik; C85084; RCJMB04_5g9; TARDBP; TDP-43; TDP43; wu:fb77f02; wu:fc52g10; XELAEV_18035188mg
UniProt ID: (Chicken) Q5ZLN5, (Mouse) Q921F2
Entrez Gene ID: (Chicken) 419453, (Mouse) 230908, (Zebrafish) 325052, (Xenopus) 380329
negative regulation of protein phosphorylation
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
mRNA processing
RNA splicing
negative regulation of gene expression
nuclear fragmentation involved in apoptotic nuclear change
negative regulation by host of viral transcription
positive regulation of transcription from RNA polymerase II promoter
regulation of cell cycle
3'-UTR-mediated mRNA stabilization
nuclear inner membrane organization
regulation of protein stability
regulation of circadian rhythm
rhythmic process
axonogenesis
skeletal muscle tissue development
neuromuscular junction development
locomotory behavior
blood vessel morphogenesis
axon extension
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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