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Invitrogen
TRIM28 Polyclonal Antibody
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图: 1 / 4
TRIM28 Antibody (PA1-20165) in ICC/IF
Immunofluorescent analysis of KAP1 in FFPE sections of human prostate carcinoma (left) and formaldelyde-fixed HeLa cells (right) using a KAP1 polyclonal antibody (Product # PA1-20165) at a dilution of 1:1000 (1µg/ml). Detection was performed using DAB (left) and a FITC-goat anti-rabbit IgG (highly cross-adsorbed) secondary antibody (green) at a dilution of 1:100 (right).
产品信息
PA1-20165
应用
建议稀释比
已发表文章
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to a portion of human tripartite motif-containing 28 (KAP1) within exon 1.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
50 µg
保存条件
4°C, do not freeze
运输条件
Wet ice
产品详细信息
PA1-20165 detects KAP1 in human samples. Expected to react with mouse samples due to 100% identity.
PA1-20165 has been successfully used in immunoprecipitation and Western blot procedures, where a band of approximately 110 kDa is detected. Whole lysate from normal 293T cells or 293T cells transiently transfected with a human KAP1 construct can be used as a positive control for this antibody.
The PA1-20165 immunogen is a synthetic peptide corresponding to a portion of human tripartite motif-containing 28 (KAP1) within exon 1 (LocusLinkID 10155).
靶标信息
The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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