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Invitrogen
GLI3 Polyclonal Antibody
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GLI3 Antibody (PA1-29595) in IHC (P)
Immunohistochemistry of GLI3 was performed in formalin fixed, paraffin embedded human glioblastoma tissue.Antigen retrieval was not required, a GLI3 Polyclonal Antibody (Product # PA1-29595) was used at 0.625 µg/mL for 1 h at RT, and a peroxidase rabbit secondary antibody was used at 1:10,000 for 45 min at RT. Localization: Gli-3 is nuclear and smooth muscle. Staining: Gli-3 as precipitated red signal with ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA1-29595
应用
建议稀释比
已发表文章
产品规格
种属反应
Fish,
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to an internal region near amino acids 30-60 of human Gli-3 protein.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2
内含物
0.01% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Expected to cross react with chicken (100% identity), human (100% identity), mouse, (94% identity), Xenopus laevis (100% identity) due to sequence homology.
靶标信息
This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GLI3 form of 190 kDa; GLI3 full-length protein; GLI3-190; GLI3FL; Transcriptional activator GLI3
基因别名: GLI3
Entrez Gene ID: (Human) 2737
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