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Invitrogen
eIF3c Polyclonal Antibody
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FIGURE: 1 / 1
eIF3c Antibody (PA1-31377) in WB
Western blot analysis of EIF3S8 using A) Whole cell lysate (15 or 50µg) from C2C12 (mouse) and L6 (rat) cells and B) Whole cell lysate (12.5µg for Input and 1 mg for IP) from HEK293 cells. Samples were probed with an EIF3S8 polyclonal antibody (Product # PA1-31377) at a dilution of 0.04µg/ml for WB (A and B) and 5µg/mg lysate for IP (B). Detection was performed using chemiluminescence with exposure times of 2-4 minutes.
Product Details
PA1-31377
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
synthetic peptide MSRFFTTGSD SESESSLSGE ELVTKPVGGN YGKQPLLLSE DEEDTKRVVR , corresponding to N terminal amino acids 1-50 of Human Eif3s8.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
PA1-31377 detects EIF3S8 from human samples.
PA1-31377 has been successfully used in immunoprecipitation, Western blot applications.
The PA1-31377 immunogen is: Synthetic peptide: MSRFFTTGSD SESESSLSGE ELVTKPVGGN YGKQPLLLSE DEEDTKRVVR , corresponding to N terminal amino acids 1-50 of Human Eif3s8.
Target Information
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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