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Invitrogen
SMAD6 Polyclonal Antibody
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图: 1 / 6
SMAD6 Antibody (PA1-41026) in ICC/IF
Immunocytochemistry analysis of SMAD6 in HepG2 cells. Samples were incubated with SMAD6 polyclonal antibody (Product # PA1-41026) using a dilution of 1:100 dilution overnight at 4°C followed by anti-rabbit DyLight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. Alpha tubulin was used as a co-stain at a 1:1,000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue)... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA1-41026
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Sheep,
Rat
已发表种属
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide made to an internal portion of human SMAD6 (between amino acids 30-150).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody is predicted to react with equine based on sequence homology.
Suggested positive control: HeLa or NIH-3T3 whole cell lysate.
Cross reacts with New World Monkey.
靶标信息
SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: MAD homolog 6; Mad homolog 7; Mothers against decapentaplegic homolog 6; mothers against DPP homolog 6; SMAD; SMAD 6; SMAD family member 6; SMAD SMAD
基因别名: b2b390Clo; MADH6; Madh7; Msmad6; SMAD6
UniProt ID: (Mouse) O35182
Entrez Gene ID: (Sheep) 101103068, (Mouse) 17130, (Rat) 367100
RNA polymerase II core promoter proximal region sequence-specific DNA binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity
ubiquitin protein ligase binding
type I transforming growth factor beta receptor binding
identical protein binding
transcription regulatory region DNA binding
metal ion binding
co-SMAD binding
I-SMAD binding
R-SMAD binding
type I activin receptor binding
molecular_function
DNA-binding transcription factor
gene-specific transcriptional regulator
transcription, DNA-templated
regulation of transcription, DNA-templated
immune response
transforming growth factor beta receptor signaling pathway
zygotic specification of dorsal/ventral axis
negative regulation of cell proliferation
negative regulation of SMAD protein complex assembly
BMP signaling pathway
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of BMP signaling pathway
cell-substrate adhesion
intracellular signal transduction
negative regulation of apoptotic process
fat cell differentiation
positive regulation of vasoconstriction
negative regulation of vasodilation
negative regulation of pathway-restricted SMAD protein phosphorylation
cardiac vascular smooth muscle cell development
coronary vasculature morphogenesis
ureteric bud development
response to laminar fluid shear stress
response to estrogen
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