Invitrogen

EZH1 Polyclonal Antibody

Name: EZH1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-41114
Availability: InStock

高级验证

1个已发表图片

2 篇参考文献

查看其他12个EZH1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

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EZH1 Antibody (PA1-41114) in WB

Western blot was performed using Anti-EZH1 Polyclonal Antibody (Product # PA1-41114) and a ~90 kDa band corresponding to Histone-lysine N-methyltransferase EZH1 was observed along with few uncharacterised band (*) in the range of 80 kDa to 20 kDa across cell lines tested. Nuclear enriched extracts (30 µg lysate) of MOLT-4 (Lane 1), HCT 116 (Lane 2), TNFa treated HCT 116 (Lane 3), THP-1 (Lane 4), HEL 92.1.7 (Lane 5) and K-562 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were... View More

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

EZH1 Antibody — Figure 7 Pharmacologic inhibition of EZH2 in SCLC inhibits growth in vitro and in vivo . ( a ) EZH1 and EZH2 are consistently expressed in a panel of SCLC PDX as measured by western blot ( b ) Ex vivo growth of the SCLC PDX LX92 is significantly inhibited by the EZH2 inhibitors EPZ-5687, GSK343 and UNC1999 as measured by resazurin conversion (two-way analysis of variance, adjusted for multiple comparisons by the method of Dunnet) ( c ) Western blot analysis of day 7 lysates from LX92 tumors treated with various EZH2 inhibitors. ( d ) In vivo growth of the SCLC PDX LX92 is significantly inhibited by the EZH2 inhibitor EPZ-6438. ( e ) Western blot analysis of end point LX92 tumor lysates treated with various EPZ-6438 treatment schedules. Tumors with growth inhibition greater than the median (TGI > median) for each treatment group were pooled separately from those with less robust response (TGI < median) to assess correlation between TGI and pharmacodynamic response. TGI, tumor growth inhibition. {TM}

Immunogen image

Immunogen Image EZH1 Polyclonal Antibody

产品信息

PA1-41114

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-4 µg/mL

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产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A portion of amino acids 350-400 of human EZH1.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2102295

产品详细信息

This antibody is 86% homologous to rat and canine.

Suggested positive control: antigen standard for EZH1 (transient overexpression lysate), human or mouse spleen.

靶标信息

EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 ).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Enhancer of zeste homolog 1; ENX-2; Histone-lysine N-methyltransferase EZH1

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基因别名: ENX-2; Enx2; EZH1; KIAA0388

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UniProt ID: (Mouse) P70351

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Entrez Gene ID: (Mouse) 14055

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功能

histone modifying enzyme

EZH1 Polyclonal Antibody

EZH1 Antibody (PA1-41114) in WB

产品信息

PA1-41114

免疫印迹 (WB)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

规格

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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