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Invitrogen
Phospho-CDK1 (Tyr15) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-CDK1 (Tyr15) Antibody (PA1-4617) in WB
Western blot of CDK1 in human T47D cells showing specific immunolabeling of a band at ~34 kDa corresponding to Phospho-CDK1 (Tyr15) polyclonal antibody (Product # PA1-4617) (Control, Lane 1). Treatment with EGF (30 ng per mL for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). Phosphospecificity is shown in lanes 3 and 4, where the immunolabeling is completely eliminated by blot treatment wit... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-4617
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Xenopus
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 of rat CDK1 conjugated to KLH
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with mouse and zebrafish based on 100% sequence homology.
This antibody is specific for the ~38 kDa cdc2 protein phosphorylated at Tyr15 in Western blots of SK-N-MC total cell extracts. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Target Information
CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ABL1; CDK1; CDK1-A; cell cycle p34 CDC2 kinase protein; Cell division control protein 2 homolog; Cell division control protein 2 homolog 1; Cell division control protein 2-A; cell division cycle 2 homolog A; cell division cycle 2 like; cell division cycle control protein 2a; Cell division protein kinase 1; Cell division protein kinase 1-A; Cyclin-dependent kinase 1; Cyclin-dependent kinase 1-A; DKFZp686L20222; KCIP-1; maturation-promoting factor; MGC111195; MGC53829; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase; p34 protein kinase 1; Protein 1054
Gene Aliases:
cdc-2; CDC2; cdc2-a; CDC28A; Cdc2a; cdc2x1.1; CDK1; cdk1-a; cdk1-b; CDKN1; MPF; p34
UniProt ID: (Mouse) P11440, (Xenopus) P35567
Entrez Gene ID: (Mouse) 12534, (Xenopus) 380246
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
RNA polymerase II carboxy-terminal domain kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
cyclin binding
Hsp70 protein binding
histone kinase activity
non-receptor serine/threonine protein kinase
protein complex assembly
protein phosphorylation
apoptotic process
cell cycle
mitotic nuclear division
mitotic G2 DNA damage checkpoint
cell aging
response to organonitrogen compound
positive regulation of gene expression
phosphorylation
histone phosphorylation
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
chromosome condensation
organ regeneration
positive regulation of protein import into nucleus, translocation
protein localization to kinetochore
negative regulation of apoptotic process
response to ethanol
positive regulation of DNA replication
positive regulation of mitotic cell cycle
cell division
positive regulation of cardiac muscle cell proliferation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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