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Invitrogen
Phospho-Dynamin 1 (Ser778) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-Dynamin 1 (Ser778) Antibody (PA1-4621) in WB
Western blot of Dynamin 1 in rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of a band at ~95 kDa corresponding to Phospho-Dynamin 1 (Ser778) polyclonal antibody (Product # PA1-4621) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1,200 units for 30 min).
Product Details
PA1-4621
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser778 of rat DNM1 conjugated to KLH
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, canine, chicken, human and mouse based on 100% sequence homology.
This antibody is specific for the ~95 kDa Dynamin protein phosphorylated at Ser778 in Western blots of rat brain lysates.
Target Information
Dynamin is a GTPase required for endocytosis of clathrin coated vesicles from the membrane. Dynamin's involvement in endocytosis was first discovered during the characterization of the temperature sensitive parylytic shibire mutation from Drosophila melanogaster. Three closely related isoforms have been identified in mammals. Dynamin1 is expressed exclusively in neurons, dynamin2 is ubiquitously expressed and dynamin3 is expressed primarily in the testes. Each of these isoforms of dynamin also has several splice variants. This suggests that each isoform/splice variant may have separate intracellular targets or function in the budding of distinct vesicle types. Common domain features of the dynamin proteins include an amino acid terminal GTPase domain, a central pleckstrin homology domain and a highly basic proline rich carboxyl terminus with several Src homology 3 (SH3) domains. These SH3 domains target the dynamin to the clathrin coated pit. Dynamin is known to interact with at least two proteins, AP2 adaptor protein complex and amphiphysin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-dynamin; D100; Dyn1; Dynamin; Dynamin BREDNM19; Dynamin I; Dynamin, brain; Dynamin-1; Dynamin1; EC 3.6.5.5; Neuronal dynamin; OTTHUMP00000215500
Gene Aliases: AI838169; Dnm; Dnm1; Ftfl; Kiaa4093; mKIAA4093
UniProt ID: (Mouse) P39053, (Rat) P21575
Entrez Gene ID: (Mouse) 13429, (Rat) 140694
nucleotide binding
GTPase activity
protein binding
GTP binding
protein C-terminus binding
hydrolase activity
protein kinase binding
D2 dopamine receptor binding
protein complex binding
identical protein binding
poly(A) RNA binding
protein dimerization activity
nitric-oxide synthase binding
membrane traffic protein
G-protein coupled receptor internalization
endocytosis
receptor-mediated endocytosis
endosome organization
sensory perception of sound
adult locomotory behavior
receptor internalization
protein tetramerization
synaptic transmission, GABAergic
clathrin-mediated endocytosis
toxin transport
positive regulation of synaptic vesicle recycling
metabolic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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