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Invitrogen
Phospho-PAK1/2/3 (Thr402) Polyclonal Antibody
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Phospho-PAK1/2/3 (Thr402) Antibody (PA1-4636) in WB
Western blot of PAK1/2/3 in rat hippocampal lysate showing specific immunolabeling of a band from ~68 kDa to ~70 kDa corresponding to Phospho-PAK1/2/3 (Thr402) polyclonal antibody (Product # PA1-4636) in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1,200 units for 30 minutes).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA1-4636
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
已发表种属
Bovine,
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 of rat PAK1-3 conjugated to KLH
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.25 mg/mL
规格
25 µg
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody is predicted to react with bovine, canine, human and mouse based on 100% sequence homology.
This antibody is specific for the ~68 kDa to ~70 kDa PAK phosphorylated at Thr402. Immunolabeling can be blocked by lambda-phosphatase treatment.
靶标信息
In mammals, there are several identified isoforms of p21-Activated Protein Kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated wit X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: activated protein kinase alpha; ADRB2; Alpha-PAK; aPAK; Beta-PAK; BPAK; CDC42/RAC effector kinase PAK-A; CDC42/RAC effector kinase PAK-B; CDKN1A; CLAP; EC 2.7.11.1; gamma pak; Gamma-PAK; hPAK3; kinase PAK1; p21 (CDKN1A)-activated kinase 1; p21 (CDKN1A)-activated kinase 2; p21 (CDKN1A)-activated kinase 3; p21-activated kinase 1; p21-activated kinase 2; p21-activated kinase 3; p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21/Cdc42/Rac1-activated kinase 3 (yeast Ste20-related); p65-PAK; p68-PAK; PAK 1; PAK alpha; PAK beta; PAK gamma; PAK-1; PAK-2; PAK-3; PAK-a; PAK-B; PAK-y; PAK65; Protein kinase MUK2; Serine/threonine-protein kinase PAK 1; Serine/threonine-protein kinase PAK 2; Serine/threonine-protein kinase PAK 3; yPAK
基因别名: 5330420P17Rik; A130002K10Rik; AI836325; AW045634; beta-PAK; D16Ertd269e; gamma-PAK; mKIAA4182; PAK-1; PAK-2; PAK-3; Pak1; Pak2; Pak3; Pak65alpha; Pak65beta; Paka; Pakb; Stk4
UniProt ID: (Rat) P35465, (Mouse) Q8CIN4, (Rat) Q64303, (Mouse) Q61036, (Rat) Q62829
Entrez Gene ID: (Mouse) 18479, (Rat) 29431, (Mouse) 224105, (Rat) 29432, (Mouse) 18481, (Rat) 29433
nucleotide binding
catalytic activity
protein kinase activity
protein serine/threonine kinase activity
protein binding
collagen binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
Rac GTPase binding
MAP kinase kinase kinase activity
protein tyrosine kinase activator activity
identical protein binding
receptor signaling protein serine/threonine kinase activity
MAP kinase kinase activity
Rho GTPase binding
SH3 domain binding
metal ion binding
GTPase binding
non-receptor serine/threonine protein kinase
response to hypoxia
positive regulation of protein phosphorylation
protein phosphorylation
exocytosis
apoptotic process
Rho protein signal transduction
regulation of mitotic cell cycle
neuromuscular junction development
metabolic process
response to organic substance
regulation of gene expression
phosphorylation
dendrite development
cell migration
amygdala development
signal transduction by protein phosphorylation
actin cytoskeleton organization
stress-activated protein kinase signaling cascade
actin cytoskeleton reorganization
activation of protein kinase activity
cellular response to insulin stimulus
regulation of actin cytoskeleton organization
positive regulation of peptidyl-serine phosphorylation
positive regulation of intracellular estrogen receptor signaling pathway
wound healing
regulation of apoptotic process
receptor clustering
regulation of MAPK cascade
positive regulation of JUN kinase activity
protein autophosphorylation
branching morphogenesis of an epithelial tube
neuron projection morphogenesis
positive regulation of stress fiber assembly
negative regulation of cell proliferation involved in contact inhibition
dendritic spine development
gamma-aminobutyric acid secretion, neurotransmission
glutamate secretion, neurotransmission
cellular response to organic cyclic compound
observational learning
regulation of long-term synaptic potentiation
MAPK cascade
activation of MAPKK activity
mitotic cell cycle
small GTPase mediated signal transduction
axonogenesis
intracellular signal transduction
peptidyl-serine phosphorylation
regulation of growth
negative regulation of apoptotic process
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of protein tyrosine kinase activity
positive regulation of extrinsic apoptotic signaling pathway
negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis
activation of MAPK activity
multicellular organism development
regulation of neuron projection development
regulation of actin filament polymerization
positive regulation of neuron apoptotic process
positive regulation of dendritic spine morphogenesis
positive regulation of DNA biosynthetic process
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