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Invitrogen
TRPML1 Polyclonal Antibody
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FIGURE: 1 / 2
TRPML1 Antibody (PA1-46474) in WB
Western blot analysis of TRPML1 in mouse brain. Samples were incubated with TRPML1 polyclonal antibody (Product # PA1-46474) using a dilution of 2.0 µg/mL in blocking buffer followed by anti-rabbit HRP secondary antibody using chemiluminescence detection reagent. Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-46474
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide made to a C-terminal portion of mouse Mucolipin 1 (within residues 500-580).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The target sequence has 85% sequence homology with monkey and 78% sequence homology with bovine.
Suggested positive control: mouse brain protein.
Target Information
Defects in Mucolipin-1 are the cause of mucolipidosis type IV (MLIV), also known as sialolipidosis. MLIV is an autosomal recessive lysosomal storage disorder characterized by severe psychomotor retardation and ophthalmologic abnormalities, including corneal opacity, retinal degeneration and strabismus. Storage bodies of lipids and water-soluble substances are seen by electron microscopy in almost every cell type of the patients. Most patients are unable to speak or walk independently and reach a maximal developmental level of 1-2 years. All patients have constitutive achlorhydia associated with a secondary elevation of serum gastrin levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MCLN1; MCOLN1 mucolipin 1; MGC7172; mucolipidin; mucolipidosis type IV protein; mucolipin 1; transient receptor potential cation channel mucolipin subfamily member 1; transient receptor potential channel mucolipin 1; transient receptor potential mucolipin 1; transient receptor potential-mucolipin 1; unnamed protein product
Gene Aliases: 2210015I05Rik; LECD; MG-2; ML1; ML4; MLIV; MST080; MSTP080; mucolipidin; TRP-ML1; TRPM-L1; TRPML1
UniProt ID: (Human) Q9GZU1, (Mouse) Q99J21
Entrez Gene ID: (Human) 57192, (Mouse) 94178, (Rat) 288371
anion channel activity
cation channel activity
calcium channel activity
potassium channel activity
sodium channel activity
iron ion transmembrane transporter activity
protein binding
identical protein binding
NAADP-sensitive calcium-release channel activity
intracellular phosphatidylinositol-3,5-bisphosphate-sensitive cation channel activity
ligand-gated calcium channel activity
cation transmembrane transporter activity
cation transport
cellular zinc ion homeostasis
calcium-mediated signaling
transferrin transport
iron ion transmembrane transport
sodium ion transmembrane transport
protein homotetramerization
calcium ion transmembrane transport
cellular response to calcium ion
cellular response to pH
potassium ion transmembrane transport
phagosome maturation
cation transmembrane transport
anion transmembrane transport
calcium ion export
positive regulation of lysosome organization
lysosome organization
endosomal transport
negative regulation of innate immune response
release of sequestered calcium ion into cytosol
autophagosome maturation
antibacterial innate immune response
negative regulation of protein localization to nucleus
positive regulation of protein localization to nucleus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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