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Invitrogen
PMCA3 ATPase Polyclonal Antibody
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PMCA3 ATPase Antibody (PA1-916) in IHC (P)
Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyc... View More
产品信息
PA1-916
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Pig,
Rat
已发表种属
Human,
Pig,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic Peptide: A(5) N S S I E F H P K P Q Q Q R E V P(22)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-916 detects calcium pump of the plasma membrane 3 (PMCA3 ATPase) from rat, human and porcine tissues.
PA1-916 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~127 kDa protein representing PMCA3a and PMCA3b ATPase from rat brain microsomal fractions.
PA1-916 immunizing peptide corresponds to amino acid residues 5-22 from rat PMCA3 ATPase protein. This sequence is completely conserved between rat and human PMCA3 ATPase.
靶标信息
The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ATPase, Ca++ transporting, plasma membrane 3; cilia and flagella associated protein 39; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 3; plasma membrane calcium pump; Plasma membrane calcium pump isoform 3; Plasma membrane calcium-transporting ATPase 3; PMCA3
基因别名: ATP2B3; CFAP39; CLA2; OPCA; PMCA3; PMCA3a; SCAX1
UniProt ID: (Human) Q16720, (Rat) Q64568
Entrez Gene ID: (Human) 492, (Pig) 100520061, (Rat) 29599
