Search

  • 联系我们
  • 货号直购

GFAP Polyclonal Antibody - FAQs

查看更多产品信息 GFAP Polyclonal Antibody - FAQs (PA316727)

3 个常见问题解答

你们提供针对神经元或神经胶质细胞的一抗吗？

请注意，只有抗神经元蛋白抗体是针对神经元细胞的。我们提供GFAP 、HuC/HuD、NMDA受体，突触蛋白I以及许多其他抗神经元蛋白抗体。更多信息以及我们的一抗搜索工具链接如下：

神经生物学抗体搜索（ (https://www.thermofisher.com/us/en/home/life-science/antibodies/primary-antibodies/neurobiology-antibodies.html)

）

The antibody I purchased from you says ''concentration not determined'' on the product label and on the product page. How do I determine the concentration?

Some antibodies are provided in non-purified formats, such as ascites fluid, tissue culture supernatant, or whole serum. These formats contain multiple proteins, which can interfere with accurate protein quantification. Traditional methods like A280 measurement or protein assays such as BCA quantify total protein but do not provide a specific concentration for the antibody in question.

Literature, such as "Antibodies" by Harlow & Lane (1988) (https://antibodiesmanual.org/index.php) (Table 8.2 - Storing and Purifying Antibodies), suggests approximate concentrations of antibodies in these solutions, as shown below. However, please note that these are rough estimates, and actual concentrations can vary based on several factors.

Serum
Total antibody concentration: 10 mg/mL
Specific antibody concentration: 0.1 to 1 mg/mL (a maximum of 10% of the antibody population is expected to be the specific antibody of interest)

Tissue Culture Supernatant (10% FBS)
Total antibody concentration: 1 mg/mL
Specific antibody concentration: 0.05 mg/mL

Tissue Culture Supernatant (serum-free)
Total antibody concentration: 0.01 to 0.05 mg/mL
Specific antibody concentration: 0.01 to 0.05 mg/mL

Ascites
Total antibody concentration: 1 to 10 mg/mL
Specific antibody concentration: 0.9 to 9 mg/mL

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

Do you offer primary antibodies that are specific for neurons or glial cells?

Please note that only antibodies against neuronal proteins are specific to neuronal cells. We carry antibodies to GFAP, HuC/HuD, NMDA receptor, synapsin I as well as many other antibodies for neuronal proteins. A link to our primary antibody search tool can be found:

Neurobiology antibody search (https://www.thermofisher.com/us/en/home/life-science/antibodies/primary-antibodies/neurobiology-antibodies.html)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.