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Invitrogen
BAG5 Polyclonal Antibody
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FIGURE: 1 / 2
BAG5 Antibody (PA5-95127) in WB
Western blot analysis of BAG5 using BAG5 Polyclonal Antibody (Product # PA5-95127). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human Hela whole cell lysates. Lane 2: human HepG2 whole cell lysates. Lane 3: rat testis tissue lysates. Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Bloc... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-95127
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human BAG5 (415-429aa QGEEKCKAARKQAVR).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human HepG2 whole cell, rat testis tissue, mouse testis tissue. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
It has been hypothesizes that the BAG-5 protein will induce the death of nigral neurons through its predicted interaction with hsp70, which will cause increased protein aggregation and cell death by disinhibition of hsp70's anti-apoptotic function. It is believed that BAG-5 will play an important role in the mechanisms of neuronal death. BAG-5 may also be of interest due to its possible role as a modulator of the hsp70/hsp40 chaperone axis or its possible interaction and coordination of localization/ modulation of other BAG containing proteins via BAG-BAG heterodimerization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BAG family molecular chaperone regulator 5; BAG-5; BAG-family molecular chaperone regulator-5 (BAG-5); Bcl-2-associated athanogene 5
Gene Aliases: 1600025G07Rik; 4930405J06Rik; Bag5
UniProt ID: (Rat) Q5QJC9, (Mouse) Q8CI32
Entrez Gene ID: (Rat) 366734, (Mouse) 70369
negative regulation of protein ubiquitination
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
negative regulation of ubiquitin-protein transferase activity
negative regulation of protein refolding
neuron death
regulation of inclusion body assembly
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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