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Invitrogen
Acetylcholinesterase Polyclonal Antibody
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FIGURE: 1 / 1
Acetylcholinesterase Antibody (PA5-95250) in WB
Western blot analysis of Acetylcholinesterase in Lane 1: rat kidney tissue lysate (50 µg), Lane 2: mouse liver tissue lysate (50 µg), Lane 3: HeLa whole cell lysate (40 µg), Lane 4: PANC whole cell lysate (40 µg), Lane 5: COLO320 whole cell lysate (40 µg). Samples were incubated with Acetylcholinesterase polyclonal antibody (Product # PA5-95250).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-95250
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ACHE (592-614aa SSYMVHWKNQFDHYSKQDRCSDL).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: Rat Kidney Tissue, Mouse Liver Tissue, HELA whole cell, PANC whole cell, COLO320 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. The Protein is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acetylcholinesterase (Cartwright blood group); apoptosis-related acetylcholinesterase; EC 3.1.1.7; glycolipid-anchored form of acetylcholinesterase; OTTHUMP00000211349; OTTHUMP00000211356; unnamed protein product; Yt blood group
Gene Aliases: ACEE; ARACHE; N-ACHE; YT
UniProt ID: (Human) P22303, (Mouse) P21836, (Rat) P37136
Entrez Gene ID: (Human) 43, (Mouse) 11423, (Rat) 83817
regulation of receptor recycling
osteoblast development
acetylcholine catabolic process
cell adhesion
synapse assembly
acetylcholine metabolic process
choline metabolic process
receptor internalization
response to insulin
positive regulation of axonogenesis
positive regulation of dendrite morphogenesis
retina development in camera-type eye
acetylcholine receptor signaling pathway
positive regulation of cold-induced thermogenesis
brain development
response to insecticide
response to nicotine
response to ethanol
response to cadmium ion
acetylcholine catabolic process in synaptic cleft
nervous system development
negative regulation of synaptic transmission, cholinergic
amyloid precursor protein metabolic process
positive regulation of protein secretion
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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