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Invitrogen
Ku80 Polyclonal Antibody
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图: 1 / 5
Ku80 Antibody (PA5-95261) in ICC/IF
Immunocytochemistry analysis of Ku80 using anti-Ku80 antibody (Product # PA5-95261). Ku80 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Ku80 antibody (Product # PA5-95261) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize usin... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-95261
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human Ku80 (649-676 aa FSEEQRFNNFLKALQEKVEIKQLNHFWE).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human A549 whole cell, human 293T whole cell, human HepG2 whole cell, rat brain tissue, mouse brain tissue. IHC: human lung cancer tissue IHC-F: human placenta tissue. ICC/IF: U20S cell. Flow: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein Ku80; DNA repair protein XRCC5; FLJ39089; HGNC:12833; Ku autoantigen protein p86 homolog; Ku autoantigen, 80kD); Ku p80; Ku-80; Ku-86; Ku80; Lupu; lupus; Nuclear factor IV; OTTHUMP00000164061; OTTHUMP00000206791; X-ray repair cross complementation (double-strand-break rejoining; X-ray repair cross complementation (double-strand-break rejoining; Ku autoantigen, 80kD); X-ray repair cross-complementing protein 5
基因别名: AI314015; G22p2; Ku80; Ku86; Kup80; Xrcc5
UniProt ID: (Mouse) P27641
Entrez Gene ID: (Rat) 363247, (Mouse) 22596
damaged DNA binding
double-stranded DNA binding
double-stranded telomeric DNA binding
ATP-dependent DNA helicase activity
protein binding
ATP binding
protein C-terminus binding
ubiquitin protein ligase binding
telomeric DNA binding
transcription regulatory region DNA binding
poly(A) RNA binding
5'-deoxyribose-5-phosphate lyase activity
nucleotide binding
DNA binding
helicase activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
DNA helicase
telomere maintenance
double-strand break repair
double-strand break repair via nonhomologous end joining
DNA recombination
brain development
cell proliferation
DNA duplex unwinding
response to drug
negative regulation of transcription, DNA-templated
positive regulation of neurogenesis
hematopoietic stem cell differentiation
cellular response to fatty acid
cellular hyperosmotic salinity response
cellular response to X-ray
DNA repair
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
