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Invitrogen
ITCH Polyclonal Antibody
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FIGURE: 1 / 2
ITCH Antibody (PA5-95264) in WB
Western blot analysis of ITCH using ITCH Polyclonal Antibody (Product # PA5-95264). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human K562 whole cell lysates. Lane 2: human Raji whole cell lysates. Lane 3: human 293T whole cell lysates. Lane 4: monkey COS-7 whole cell lysates. Lane 5: human HepG2 whole cell lysates. Lane 6: human A549 whole cell lysates. Lane 7: rat C6 whole cell... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-95264
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human ITCH/AIP4 (410-437aa AMQQFNQRFIYGNQDLFATSQSKEFDPL).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human Raji whole cell, human 293T whole cell, monkey COS-7 whole cell, human HepG2 whole cell, human A549 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell. Flow: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dJ468O1.1; E3 ubiquitin-protein ligase Itchy; E3 ubiquitin-protein ligase Itchy homolog; HECT-type E3 ubiquitin transferase Itchy homolog; itch E3 ubiquitin ligase; itchy E3 ubiquitin protein ligase homolog; itchy, E3 ubiquitin protein ligase
Gene Aliases: 6720481N21Rik; 8030492O04Rik; A130065M08; AIP4; C230047C07Rik; Itch
UniProt ID: (Mouse) Q8C863
Entrez Gene ID: (Mouse) 16396, (Rat) 311567
ubiquitin-protein transferase activity
protein binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
ribonucleoprotein complex binding
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
CXCR chemokine receptor binding
ubiquitin protein ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein polyubiquitination
immune system process
positive regulation of T cell anergy
ubiquitin-dependent protein catabolic process
apoptotic process
protein ubiquitination
negative regulation of NF-kappaB transcription factor activity
protein K29-linked ubiquitination
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
innate immune response
positive regulation of protein catabolic process
negative regulation of JNK cascade
negative regulation of alpha-beta T cell proliferation
negative regulation of defense response to virus
defense response to virus
protein K63-linked ubiquitination
protein K48-linked ubiquitination
regulation of protein deubiquitination
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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