Invitrogen

NOVA1 Polyclonal Antibody

Name: NOVA1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-95571
Availability: InStock

查看其他13个NOVA1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (9)

NOVA1 Antibody in Immunocytochemistry (ICC/IF)

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NOVA1 Antibody (PA5-95571) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of NOVA1 in U2OS cell using NOVA1 Polyclonal Antibody (Product # PA5-95571). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 2 µg/mL and mouse anti-Beta Tubulin antibody overnight at 4°C. DyLight 488 conjugated goat anti-rabbit IgG and DyLight 594 conjugated goat anti-mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°... View More

NOVA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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产品信息

PA5-95571

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

免疫组化（冰冻） (IHC (F))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Nova1 recombinant protein (Position: T416-V509).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose, 0.9mg NaCl, 0.2mg Na2PO4

内含物

0.01mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2807373

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human PC-3 whole cell, human A549 whole cell, human U87 whole cell, human THP-1 whole cell, rat brain tissue, mouse brain tissue. IHC: human breast cancer tissue, human colorectal adenocarcinoma tissue, human liver cancer tissue, mouse brain tissue, mouse brain tissue, rat brain tissue, rat brain tissue IHC-F: human placenta tissue. ICC/IF: A549 cell, U2OS cell. Flow: U87 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described.

仅用于科研。不用于诊断过程。未经明确授权不得转售。