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Invitrogen
LARS Polyclonal Antibody
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FIGURE: 1 / 1
LARS Antibody (PA5-95627) in WB
Western blot analysis of LARS in Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH3T3 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at roo... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-95627
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Leucyl tRNA synthetase/LARS recombinant protein (Q628-Q855).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HL-60 whole cell, human K562 whole cell, human THP-1 whole cell, human Caco-2 whole cell, rat liver tissue, mouse brain tissue, mouse liver tissue, mouse NIH3T3 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. Alternatively spliced transcript variants of this gene have been found; however, their full-length nature is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytoplasmic leucyl-tRNA synthetase; cytosolic leucyl-tRNA synthetase; leucine translase; leucine tRNA ligase 1, cytoplasmic; leucine-tRNA ligase; leucyl-tRNA synthetase, cytoplasmic; PIG57; proliferation-inducing gene 44; unnamed protein product
Gene Aliases: 2310045K21Rik; 3110009L02Rik; cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS; LEUS; LFIS; LRS; PIG44; RNTLS
UniProt ID: (Human) Q9P2J5, (Mouse) Q8BMJ2
Entrez Gene ID: (Human) 51520, (Mouse) 107045, (Rat) 291624
tRNA aminoacylation for protein translation
glutaminyl-tRNA aminoacylation
leucyl-tRNA aminoacylation
regulation of cell size
positive regulation of TOR signaling
cellular response to amino acid starvation
cellular response to amino acid stimulus
cellular response to leucine
aminoacyl-tRNA metabolism involved in translational fidelity
positive regulation of TORC1 signaling
cellular response to leucine starvation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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