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Invitrogen
INSIG1 Polyclonal Antibody
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图: 1 / 3
INSIG1 Antibody (PA5-100150) in ICC/IF
Immunofluorescent analysis of INSIG1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with INSIG1 polyclonal antibody (Product # PA5-100150) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-100150
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human INSIG1(Accession O15503), corresponding to amino acid residues W127-A177.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total INSIG1.
靶标信息
Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: INSIG-1; INSIG-1 membrane protein; Insulin-induced gene 1 protein; MGC1405
基因别名: 1810013C12Rik; Insig-1; INSIG1
UniProt ID: (Mouse) Q8BGI3
Entrez Gene ID: (Mouse) 231070
lipid metabolic process
triglyceride metabolic process
cholesterol biosynthetic process
response to sterol depletion
steroid metabolic process
cholesterol metabolic process
negative regulation of steroid biosynthetic process
sterol biosynthetic process
SREBP signaling pathway
inner ear morphogenesis
middle ear morphogenesis
negative regulation of fat cell differentiation
negative regulation of fatty acid biosynthetic process
palate development
cranial suture morphogenesis
negative regulation of cargo loading into COPII-coated vesicle
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