Invitrogen

BBX Polyclonal Antibody

查看其他7个BBX抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (3)

BBX Antibody in Immunocytochemistry (ICC/IF)

BBX Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

BBX Antibody (PA5-100221) in ICC/IF

Immunofluorescent analysis of BBX in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and BBX polyclonal antibody (Product # PA5-100221) using a dilution of 1:100 (primary, 1 hr, 37°C), 1:200 (tubulin), and followed by goat anti-mouse Alexa Fluor 594 and goat anti-rabbit IgG (H+L) Alexa Fluor 488 conjugated.

BBX Antibody in Immunocytochemistry (ICC/IF)

BBX Antibody in Western Blot (WB)

BBX Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

BBX Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of BBX in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and BBX polyclonal antibody (Product # PA5-100221) using a dilution of 1:100 (primary, 1 hr, 37°C), 1:200 (tubulin), and followed by goat anti-mouse Alexa Fluor 594 and goat anti-rabbit IgG (H+L) Alexa Fluor 488 conjugated.

BBX Antibody in Western Blot (WB) — Western blot analysis of BBX in HepG2 cell lysate (left lane: treated with blocking peptide). Samples were incubated with BBX polyclonal antibody (Product # PA5-100221).

BBX Antibody in Western Blot (WB) — Western blot analysis of BBX in PC12 cell lysate (left lane: treated with blocking peptide). Samples were incubated with BBX polyclonal antibody (Product # PA5-100221).

Immunogen image

BBX Polyclonal Antibody

Immunogen Image BBX Polyclonal Antibody

产品信息

PA5-100221

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human BBX(Accession Q8WY36), corresponding to amino acid residues D131-L181.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total BBX.

靶标信息

BBX gene ontology annotations related to this gene include DNA binding.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Bobby sox homolog; HMG box transcription factor BBX; HMG box-containing protein 2

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基因别名: 5530401J07Rik; 5730403O13Rik; BBX; HBP2

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UniProt ID: (Mouse) Q8VBW5

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Entrez Gene ID: (Mouse) 70508, (Rat) 303970

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DNA binding molecular_function DNA-binding transcription factor

transcription, DNA-templated regulation of transcription, DNA-templated bone development biological_process