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Invitrogen
MED12 Polyclonal Antibody
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图: 1 / 5
MED12 Antibody (PA5-100279) in ICC/IF
Immunocytochemistry analysis of MED12 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MED12 polyclonal antibody (Product # PA5-100279) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-100279
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human MED12(Accession Q93074), corresponding to amino acid residues S671-T721.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total MED12.
靶标信息
The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; Mediator complex subunit 12; Mediator of RNA polymerase II transcription subunit 12; mediator of RNA polymerase II transcription, subunit 12 homolog; OPA-containing protein; OPA-containing protein 1; Thyroid hormone receptor-associated protein complex 230 kDa component; Trap230; trinucleotide repeat containing 11 (THR-associated protein); trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit); Trinucleotide repeat-containing gene 11 protein
基因别名: 230kDa; ARC240; CAGH45; HOPA; KIAA0192; MED12; Mopa; OPA-1; TNRC11; TRAP230
UniProt ID: (Mouse) A2AGH6
Entrez Gene ID: (Rat) 679693, (Mouse) 59024
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription cofactor activity
transcriptional activator activity, RNA polymerase II transcription factor binding
chromatin binding
transcription cofactor activity
transcription coactivator activity
receptor activity
beta-catenin binding
protein C-terminus binding
transcription factor binding
protein domain specific binding
thyroid hormone receptor binding
ubiquitin protein ligase activity
protein binding
general transcription factor
neural tube closure
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
heart development
oligodendrocyte development
Schwann cell development
protein ubiquitination
stem cell population maintenance
negative regulation of Wnt signaling pathway
intracellular steroid hormone receptor signaling pathway
androgen receptor signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
canonical Wnt signaling pathway
Wnt signaling pathway, planar cell polarity pathway
axis elongation involved in somitogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
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