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Invitrogen
MED10 Polyclonal Antibody
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FIGURE: 1 / 6
MED10 Antibody (PA5-100608) in ICC/IF
Immunocytochemistry analysis of MED10 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MED10 polyclonal antibody (Product # PA5-100608) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-100608
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MED10(Accession Q9BTT4), corresponding to amino acid residues E10-D60.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total MED10.
Target Information
MED10 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mediator of RNA polymerase II transcription, subunit 10 homolog (NUT2, S. cerevisiae); transformation-related gene 17 protein; transformation-related gene 20 protein; TRG-17; TRG-20; unnamed protein product
Gene Aliases: D13Wsu50e; L6; NUT2; RGD1310128; TRG20
UniProt ID: (Human) Q9BTT4, (Mouse) Q9CXU0
Entrez Gene ID: (Human) 84246, (Mouse) 28077, (Rat) 290939
regulation of transcription from RNA polymerase II promoter
protein ubiquitination
stem cell population maintenance
positive regulation of transcription elongation from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
RNA polymerase II transcriptional preinitiation complex assembly
positive regulation of transcription initiation from RNA polymerase II promoter
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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