Invitrogen

OTX1 Polyclonal Antibody

查看其他10个OTX1抗体

问题与答案

问题与答案

问题与答案

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抗体检测数据 (2)

OTX1 Antibody in Immunocytochemistry (ICC/IF)

OTX1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 2

OTX1 Antibody (PA5-101015) in ICC/IF

Immunofluorescent analysis of OTX1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and OTX1 polyclonal antibody (Product # PA5-101015) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

OTX1 Antibody in Immunocytochemistry (ICC/IF)

OTX1 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

OTX1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of OTX1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and OTX1 polyclonal antibody (Product # PA5-101015) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

OTX1 Antibody in Western Blot (WB) — Western blot analysis of OTX1 in mouse brain tissue lysate (left lane: treated with blocking peptide). Samples were incubated with OTX1 polyclonal antibody (Product # PA5-101015).

Immunogen image

OTX1 Polyclonal Antibody

Immunogen Image OTX1 Polyclonal Antibody

产品信息

PA5-101015

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human OTX1(Accession P32242), corresponding to amino acid residues T237-S287.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total Otx1.

靶标信息

Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Homeobox protein OTX1; Orthodenticle homolog 1; OTX

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基因别名: A730044F23Rik; jv; Otx-1; OTX1; OTX1X

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UniProt ID: (Mouse) P80205, (Rat) Q63410

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Entrez Gene ID: (Mouse) 18423, (Rat) 25646

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RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding homeodomain transcription factor

regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development anterior/posterior pattern specification metencephalon development forebrain development midbrain development inner ear morphogenesis positive regulation of transcription from RNA polymerase II promoter diencephalon morphogenesis