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Invitrogen
NCBP2 Polyclonal Antibody
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图: 1 / 2
NCBP2 Antibody (PA5-101122) in ICC/IF
Immunocytochemistry analysis of NCBP2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NCBP2 polyclonal antibody (Product # PA5-101122) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-101122
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human NCBP2(Accession P52298), corresponding to amino acid residues M1-T51.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total NCBP2.
靶标信息
The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 20 kDa nuclear cap-binding protein; CBC2; CBP20; Cell proliferation-inducing gene 55 protein; NCBP 20 kDa subunit; NCBP-interacting protein 1; NIP1; nuclear cap binding protein subunit 2, 20kDa; Nuclear cap-binding protein subunit 2; PIG55
基因别名: 20kDa; 5930413E18Rik; AA536802; AI843301; C79367; CBP20; NCBP2; PIG55
UniProt ID: (Mouse) Q9CQ49
Entrez Gene ID: (Mouse) 68092
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
mRNA splicing, via spliceosome
7-methylguanosine mRNA capping
mRNA processing
regulation of translation
regulation of translational initiation
transport
RNA splicing
gene silencing by RNA
mRNA cis splicing, via spliceosome
mRNA transport
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