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Invitrogen
CNOT7 Polyclonal Antibody
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图: 1 / 2
CNOT7 Antibody (PA5-101270) in ICC/IF
Immunocytochemistry analysis of CNOT7 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CNOT7 polyclonal antibody (Product # PA5-101270) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-101270
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human CNOT7(Accession Q9UIV1), corresponding to amino acid residues E243-S285.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total CNOT7.
靶标信息
CNOT7 (CCR4-NOT transcription complex subunit 7) has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. CNOT7 is a catalytic component of the CCR4-NOT complex, which is one of the major cellular mRNA deadenylases, and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation, and general transcription regulation. During miRNA-mediated repression, the complex seems to act as a transcriptional repressor during translational initiation, as well. Additional complex functions may be a consequence of its influence on mRNA expression. CNOT7 associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BTG1-binding factor 1; CAF-1; Caf1a; catabolite repressor protein (CCR4)-associative factor 1; CCR4-associated factor 1; CCR4-NOT transcription complex subunit 7; POP2 homolog
基因别名: AU022737; CAF-1; CAF1; CNOT7; Pop2
UniProt ID: (Mouse) Q60809
Entrez Gene ID: (Mouse) 18983
nuclear-transcribed mRNA poly(A) tail shortening
deadenylation-dependent decapping of nuclear-transcribed mRNA
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of translation
positive regulation of cell proliferation
negative regulation of cell proliferation
negative regulation of gene expression
gene silencing by RNA
cytoplasmic mRNA processing body assembly
exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay
positive regulation of transcription from RNA polymerase II promoter
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
positive regulation of mRNA catabolic process
RNA phosphodiester bond hydrolysis, exonucleolytic
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
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