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Invitrogen
Carbonic Anhydrase VA Polyclonal Antibody
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FIGURE: 1 / 3
Carbonic Anhydrase VA Antibody (PA5-101492) in ICC/IF
Immunocytochemistry analysis of Carbonic Anhydrase VA in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Carbonic Anhydrase VA polyclonal antibody (Product # PA5-101492) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an Al... View More
Product Details
PA5-101492
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CA5A(Accession P35218), corresponding to amino acid residues I180-Y230.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CA5A.
Target Information
Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA5A gene encodes for the CA V protein which is expressed in the mitochondrial matrix in liver tissues, but can also be detected in many other tissues. In ureagenesis, the CA V protein may be involved in providing bicarbonate to carbamoyl phosphate synthetase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CA Y; CA-VA; Carbonate dehydratase VA; carbonic anhydrase 5, mitochondrial; Carbonic anhydrase 5A, mitochondrial; carbonic anhydrase 5b, mitochondrial; carbonic anhydrase V, mitochondrial; Carbonic anhydrase VA; carbonic anhydrase VA, mitochondrial; carbonic dehydratase
Gene Aliases: CA5; CA5A; CA5AD; Car5; Car5a; CAV; CAVA; GS1-21A4.1
UniProt ID: (Human) P35218, (Mouse) P23589
Entrez Gene ID: (Human) 763, (Mouse) 12352
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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