Invitrogen

SIX1 Polyclonal Antibody

查看其他7个SIX1抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (2)

SIX1 Antibody in Immunocytochemistry (ICC/IF)

SIX1 Antibody in Immunocytochemistry (ICC/IF)

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SIX1 Antibody (PA5-101643) in ICC/IF

Immunocytochemistry analysis of SIX1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SIX1 polyclonal antibody (Product # PA5-101643) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

SIX1 Antibody in Immunocytochemistry (ICC/IF)

SIX1 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

SIX1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of SIX1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SIX1 polyclonal antibody (Product # PA5-101643) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

SIX1 Antibody in Western Blot (WB) — Western blot analysis of SIX1 in NIH/3T3 cells. Samples were incubated with SIX1 polyclonal antibody (Product # PA5-101643).

Immunogen image

SIX1 Polyclonal Antibody

Immunogen Image SIX1 Polyclonal Antibody

产品信息

PA5-101643

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human SIX1(Accession Q15475), corresponding to amino acid residues V111-T161.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total SIX1.

靶标信息

The protein encoded by this gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Homeobox protein SIX1; Sine oculis homeobox homolog 1; sine oculis-related homeobox 1 homolog

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基因别名: BB138287; SIX1

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UniProt ID: (Mouse) Q62231

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Entrez Gene ID: (Mouse) 20471

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RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding transcription regulatory region DNA binding homeodomain transcription factor

negative regulation of transcription from RNA polymerase II promoter ureteric bud development branching involved in ureteric bud morphogenesis organ induction kidney development outflow tract morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter apoptotic process multicellular organism development pattern specification process skeletal muscle tissue development sensory perception of sound regulation of synaptic growth at neuromuscular junction regulation of gene expression regulation of skeletal muscle satellite cell proliferation facial nerve morphogenesis neurogenesis epithelial cell differentiation thyroid gland development olfactory placode formation regulation of protein localization protein localization to nucleus aorta morphogenesis inner ear morphogenesis middle ear morphogenesis negative regulation of apoptotic process negative regulation of neuron apoptotic process tongue development regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter thymus development neuron fate specification generation of neurons embryonic cranial skeleton morphogenesis embryonic skeletal system morphogenesis skeletal system morphogenesis inner ear development anatomical structure development regulation of epithelial cell proliferation myoblast migration pharyngeal system development myotome development fungiform papilla morphogenesis trigeminal ganglion development otic vesicle development renal system development metanephric mesenchyme development regulation of branch elongation involved in ureteric bud branching positive regulation of ureteric bud formation mesonephric tubule formation ureter smooth muscle cell differentiation positive regulation of secondary heart field cardioblast proliferation cochlea morphogenesis positive regulation of branching involved in ureteric bud morphogenesis negative regulation of branching involved in ureteric bud morphogenesis positive regulation of mesenchymal cell proliferation involved in ureter development regulation of skeletal muscle cell differentiation