Invitrogen

ARHGAP11A Polyclonal Antibody

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1 篇参考文献

查看其他6个ARHGAP11A抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (2)
已发表图片 (1)

ARHGAP11A Antibody in Immunocytochemistry (ICC/IF)

ARHGAP11A Antibody in Immunocytochemistry (ICC/IF)

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ARHGAP11A Antibody (PA5-101840) in ICC/IF

Immunocytochemistry analysis of ARHGAP11A in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ARHGAP11A polyclonal antibody (Product # PA5-101840) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated g... View More

ARHGAP11A Antibody in Immunocytochemistry (ICC/IF)

ARHGAP11A Antibody in Western Blot (WB)

ARHGAP11A Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

ARHGAP11A Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of ARHGAP11A in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ARHGAP11A polyclonal antibody (Product # PA5-101840) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

ARHGAP11A Antibody in Western Blot (WB) — Western blot analysis of ARHGAP11A in hybridoma cell lysate (left lane: treated with blocking peptide). Samples were incubated with ARHGAP11A polyclonal antibody (Product # PA5-101840).

ARHGAP11A Antibody in Western Blot (WB) — High ARHGAP11A level is positively correlated with the malignancy of ccRCC patients. ( A , B ) ARHGAP11A mRNA level was upregulated in both ccRCC tissues compared with adjacent normal tissues ( A , GEO GSE53757 data) and RCC cells compared with immortalized renal epithelial HK2 cells ( B , CCLE data). ( C ) WB verified that ARHGAP11A protein level was upregulated in RCC cells compared with HK2 cells. beta-actin was used as a loading control. ( D - F ) ARHGAP11A mRNA level was gradually upregulated as AJCC stage ( D ), T stage ( E ), and Fuhrman grade ( F ) progressed. ( G - I ) ARHGAP11A mRNA level was positively correlated with lymph node metastasis ( G ), distant metastasis ( H ), and recurrence ( I ) in ccRCC patients. ( J ) Enrichment plot of gene expression signature for relapse (KAUFFMANN_MELANOMA_RELAPSE_UP) obtained by GSEA according to ARHGAP11A mRNA levels. The ccRCC samples from the TCGA_KIRC database were divided into high and low ARHGAP11A expression groups according to the median value of ARHGAP11A RNA-seq quantification results. ** p < 0.01, **** p < 0.0001.

Immunogen image

ARHGAP11A Polyclonal Antibody

Immunogen Image ARHGAP11A Polyclonal Antibody

产品信息

PA5-101840

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human ARHGAP11A(Accession Q6P4F7), corresponding to amino acid residues Q957-H1007.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total ARHGAP11A.

靶标信息

ARHGAP11A is a protein coding gene. Gene Ontology (GO) annotations related to this gene include signal transduction.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: GAP (1-12); KIAA0013; Rho GTPase-activating protein 11A; Rho-type GTPase-activating protein 11A; unnamed protein product

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基因别名: 6530401L14Rik; ARHGAP11A; GAP (1-12); KIAA0013; mKIAA0013

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UniProt ID: (Mouse) Q80Y19

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Entrez Gene ID: (Human) 9824, (Mouse) 228482

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GTPase activator activity molecular_function G-protein modulator protein-binding activity modulator GTPase-activating protein

signal transduction positive regulation of GTPase activity regulation of small GTPase mediated signal transduction