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Invitrogen
GPR115 Polyclonal Antibody
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FIGURE: 1 / 3
GPR115 Antibody (PA5-102083) in ICC/IF
Immunocytochemistry analysis of GPR115 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with GPR115 polyclonal antibody (Product # PA5-102083) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-102083
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ADGRF4(Accession Q8IZF3), corresponding to amino acid residues R660-G695.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total GPR115.
Target Information
G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR115 is a member of this family (subfamily Orphan-B). ESTs have been isolated from breast, heart, and placenta libraries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ38076; G protein coupled receptor Gpr115; G protein-coupled receptor 115; G-protein coupled receptor PGR18; probable G-protein coupled receptor 115; seven transmembrane helix receptor; similar to KIAA0758 protein; unnamed protein product
Gene Aliases: 4632435A09Rik; GPR115; PGR18
UniProt ID: (Human) Q8IZF3, (Mouse) Q9D2L6
Entrez Gene ID: (Human) 221393, (Mouse) 78249
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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