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Invitrogen
POLR2I Polyclonal Antibody
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POLR2I Antibody (PA5-102504) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded POLR2I in rat kidney tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with POLR2I polyclonal antibody (Product # PA5-102504) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-102504
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human POLR2I(Accession P36954), corresponding to amino acid residues Y54-A104.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total POLR2I.
靶标信息
POLR2I is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. POLR2I has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests. This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DNA-directed RNA polymerase II subunit I; DNA-directed RNA polymerase II subunit RPB9; hRPB14.5; polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa; RNA polymerase II 14.5 kDa subunit; RNA polymerase II subunit B9; RPB14.5; RPB9
基因别名: 2810002B19Rik; POLR2I
UniProt ID: (Mouse) P60898
Entrez Gene ID: (Rat) 292778, (Mouse) 69920
maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter
transcription-coupled nucleotide-excision repair
transcription initiation from RNA polymerase II promoter
biological_process
transcription, DNA-templated
transcription from RNA polymerase II promoter
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