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Invitrogen
WNK3 Polyclonal Antibody
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FIGURE: 1 / 6
WNK3 Antibody (PA5-102806) in ICC/IF
Immunocytochemistry analysis of WNK3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with WNK3 polyclonal antibody (Product # PA5-102806) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-102806
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human WNK3(Accession Q9BYP7), corresponding to amino acid residues S1616-L1666.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total WNK3.
Target Information
WNK3 is a serine/threonine kinase involved in signal transduction and cellular communication. Members of the 'with no lysine' kinase family, such as WNK3, are serine-threonine protein kinases that lack the almost invariant catalytic lysine in subdomain II, which is important for binding ATP in the catalytic site. Instead, these kinases have a conserved lysine in subdomain I that is thought to provide this function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PRKWNK 3; Protein kinase lysine-deficient 3; Protein kinase with no lysine 3; Serine/threonine-protein kinase WNK3; WNK 3; WNK lysine deficient protein kinase 3, pseudogene
Gene Aliases: KIAA1566; PRKWNK3; RGD1563131; WNK3; Wnk3-ps
UniProt ID: (Mouse) Q80XP9
Entrez Gene ID: (Mouse) 279561, (Rat) 317420
protein phosphorylation
positive regulation of sodium ion transport
positive regulation of peptidyl-threonine phosphorylation
phosphorylation
positive regulation of ion transmembrane transporter activity
negative regulation of apoptotic process
protein autophosphorylation
positive regulation of calcium ion transport
positive regulation of establishment of protein localization to plasma membrane
negative regulation of pancreatic juice secretion
regulation of ion homeostasis
positive regulation of sodium ion transmembrane transporter activity
positive regulation of rubidium ion transport
positive regulation of rubidium ion transmembrane transporter activity
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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