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Invitrogen
PIKFYVE Polyclonal Antibody
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FIGURE: 1 / 2
PIKFYVE Antibody (PA5-103242) in IHC (P)
Immunohistochemistry analysis of PIKFYVE in mouse heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with PIKFYVE polyclonal antibody (Product # PA5-103242) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-103242
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PIKFYVE(Accession Q9Y2I7), corresponding to amino acid residues T283-N333.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total PIP5K.
Target Information
PIKFYVE belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol. The content of phosphatidylinositol 3,5-bisphosphateP2) in endosomal membranes changes dynamically with fission and fusion events that generate or absorb intracellular transport vesicles. PIKFYVE is the PtdIns(3,5)P2-producing component of a trimolecular complex that tightly regulates the level of PtdIns(3,5)P2. Other components of this complex are the PIKFYVE activator VAC14 and the PtdIns(3,5)P2 phosphatase FIG4. In addition to its phosphoinositide 5-kinase activity, PIKFYVE also has protein kinase activity. For a review, see Shisheva.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1-phosphatidylinositol 3-phosphate 5-kinase; 1-phosphatidylinositol-3-phosphate 5-kinase; FYVE finger-containing phosphoinositide kinase; p235; Phosphatidylinositol 3-phosphate 5-kinase; Phosphatidylinositol 3-phosphate 5-kinase type III; phosphatidylinositol-3-phosphate 5-kinase type III; phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III; phosphatidylinositol-4-phosphate 5-kinase, type III; PIKfyve; PIPkin-III; PtdIns(4)P-5-kinase; Serine-protein kinase PIKFYVE; type III PIP kinase
Gene Aliases: 5230400C17Rik; Fab1; Kiaa0981; p235; Pikfyve; Pip5k; Pip5k3; Pipk5k3; PipkIII
UniProt ID: (Mouse) Q9Z1T6
Entrez Gene ID: (Mouse) 18711
nucleotide binding
1-phosphatidylinositol-3-phosphate 5-kinase activity
protein binding
ATP binding
zinc ion binding
kinase activity
phosphatidylinositol phosphate kinase activity
1-phosphatidylinositol-4-phosphate 5-kinase activity
transferase activity
metal ion binding
kinase
transferase
metabolite interconversion enzyme
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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