Invitrogen

NCLN Polyclonal Antibody

查看其他9个NCLN抗体

问题与答案

问题与答案

问题与答案

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抗体检测数据 (3)

NCLN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NCLN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NCLN Antibody (PA5-104276) in IHC (P)

Immunohistochemistry analysis of NCLN in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with NCLN polyclonal antibody (Product # PA5-104276) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

NCLN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NCLN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NCLN Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

NCLN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NCLN in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with NCLN polyclonal antibody (Product # PA5-104276) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

NCLN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NCLN in rat muscle tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with NCLN polyclonal antibody (Product # PA5-104276) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

NCLN Antibody in Western Blot (WB) — Western blot analysis of NCLN in mouse heart tissue lysate (left lane: treated with blocking peptide). Samples were incubated with NCLN polyclonal antibody (Product # PA5-104276).

Immunogen image

NCLN Polyclonal Antibody

Immunogen Image NCLN Polyclonal Antibody

产品信息

PA5-104276

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human NCLN(Accession Q969V3), corresponding to amino acid residues A34-C84.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total Nicalin.

靶标信息

May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning. [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: BOS complex subunit NCLN; Nicalin; nicalin homolog; Nicastrin-like protein

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基因别名: 3100002P13Rik; AW120537; NCLN; RGD1309355

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UniProt ID: (Mouse) Q8VCM8, (Rat) Q5XIA1

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Entrez Gene ID: (Mouse) 103425, (Rat) 314648

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molecular_function

regulation of signal transduction protein destabilization regulation of protein complex assembly