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Invitrogen
Phospho-IKK alpha (Thr23) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-IKK alpha (Thr23) Antibody (PA5-104695) in ICC/IF
Immunocytochemistry analysis of Phospho-IKK alpha (Thr23) in Hela cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-IKK alpha (Thr23) polyclonal antibody (Product # PA5-104695) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugat... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104695
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CHUK(Accession O15111), corresponding to amino acid residues around phosphorylated Thr23.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of IKK alpha only when phosphorylated at Threonine 23.
Target Information
CHUK (IKK alpha) is a serine/threonine kinase involved in signal transduction and cellular communication. It belongs to the cytokine activated protein complex that is an inhibitor of the NF-kappa B complex. IKK alpha along with IKK beta and a regulatory protein NEMO (IKK gamma) phosphorylates IkB proteins which targets the later for ubiquitin mediated degradation. It is involved with several cellular processes with extensive studies on its role in inflammation and cell survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: conserved helix-loop-helix ubiquitous kinase; EC 2.7.11.10; FLJ40509; I kappa B kinase 1; I-kappa-B kinase 1; I-kappa-B kinase 2; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; ikappaB kinase; IkappaB kinase alpha; IkB kinase alpha subunit; IKBKB; IKK-a kinase; IKK-B; IKK-beta; IKK2; IKKB; kinase beta; MGC131801; NFKBIKB; nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NFkappaB inhibitor kinase alpha; protein kinase; transcription factor 16; unnamed protein product
Gene Aliases: BPS2; Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha; IKK-1; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16
UniProt ID: (Human) O15111
Entrez Gene ID: (Human) 1147, (Mouse) 12675, (Rat) 309361
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
IkappaB kinase activity
protein homodimerization activity
macromolecular complex binding
protein heterodimerization activity
scaffold protein binding
transferrin receptor binding
identical protein binding
nucleotide binding
morphogenesis of an epithelial sheet
regulation of transcription from RNA polymerase II promoter
integrin-mediated signaling pathway
I-kappaB kinase/NF-kappaB signaling
I-kappaB phosphorylation
lactation
osteoclast differentiation
positive regulation of interferon-alpha production
tumor necrosis factor-mediated signaling pathway
mammary gland epithelial cell proliferation
toll-like receptor 4 signaling pathway
response to muscle stretch
NIK/NF-kappaB signaling
odontogenesis of dentin-containing tooth
positive regulation of I-kappaB kinase/NF-kappaB signaling
skin development
positive regulation of transcription from RNA polymerase II promoter
striated muscle cell differentiation
defense response to virus
mammary gland alveolus development
response to cholecystokinin
cellular response to tumor necrosis factor
cellular response to virus
pattern recognition receptor signaling pathway
skeletal muscle contraction
inflammatory response
immune response
response to xenobiotic stimulus
response to virus
response to toxic substance
anatomical structure morphogenesis
response to acetate
response to hydroperoxide
response to amino acid
innate immune response
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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