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Invitrogen
Phospho-JunB (Ser259) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-JunB (Ser259) Antibody (PA5-104819) in ICC/IF
Immunofluorescent analysis of Phospho-JunB (Ser259) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-JunB (Ser259) polyclonal antibody (Product # PA5-104819) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104819
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human JUNB(Accession P17275), corresponding to amino acid residues around phosphorylated Ser259.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of JunB only when phosphorylated at Serine 259.
Target Information
JunB, a member of the Jun cellular oncogene family, is a basic region, leucine zipper transcription factor. It is involved in regulating gene activity following the primary growth factor response. JunB binds to the DNA sequence 5'-TGA[CG]TCA-3'. Cellular oncogenes play critical roles in cellular communication pathways that regulate growth, development, and differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Jun B; Jun-B oncogene; MyD21; Oncogene Jun-B; Transcription factor AP-1 subunit JunB; transcription factor jun-B; Transcription factor JunB
Gene Aliases: Jun-b; JUNB
UniProt ID: (Rat) P24898, (Mouse) P09450
Entrez Gene ID: (Rat) 24517, (Mouse) 16477
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
protein heterodimerization activity
transcription coactivator activity
protein binding
transcription factor binding
basic leucine zipper transcription factor
vasculogenesis
osteoblast differentiation
in utero embryonic development
trophectodermal cell differentiation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
female pregnancy
response to light stimulus
response to mechanical stimulus
cellular process
response to organic cyclic compound
osteoclast differentiation
response to progesterone
cellular response to hormone stimulus
osteoblast proliferation
response to cytokine
response to drug
response to peptide hormone
positive regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
decidualization
response to corticosterone
response to cAMP
regulation of cell cycle
embryonic process involved in female pregnancy
labyrinthine layer blood vessel development
cellular response to calcium ion
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
response to radiation
regulation of cell death
response to lipopolysaccharide
regulation of cell proliferation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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