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Invitrogen
Phospho-Shc (Tyr349) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-Shc (Tyr349) Antibody (PA5-104856) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-Shc (Tyr349) in human Lymphoma tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Shc (Tyr349) polyclonal antibody (Product # PA5-104856) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104856
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SHC1(Accession P29353), corresponding to amino acid residues around phosphorylated Tyr349.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of SHC only when phosphorylated at Tyrosine 349.
Target Information
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ26504; OTTHUMP00000035472; RP11-307C12.1; SH2 domain protein C1; SHC (Src homology 2 domain-containing) transforming protein 1; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; src homology 2 domain-containing transforming protein C1; Src homology 2 domain-containing-transforming protein C1
Gene Aliases: p66; P66shc; SHC; SHC1; SHCA
UniProt ID: (Rat) Q5M824, (Mouse) P98083
Entrez Gene ID: (Rat) 85385, (Mouse) 20416
phosphotyrosine binding
epidermal growth factor receptor binding
insulin receptor binding
insulin-like growth factor receptor binding
neurotrophin TRKA receptor binding
protein binding
protein kinase binding
receptor tyrosine kinase binding
protein complex binding
ephrin receptor binding
phosphoprotein binding
protein phosphatase 2A binding
scaffold/adaptor protein
MAPK cascade
activation of MAPK activity
angiogenesis
response to hypoxia
epidermal growth factor receptor signaling pathway
heart development
aging
insulin receptor signaling pathway
response to toxic substance
response to organic cyclic compound
single organismal cell-cell adhesion
actin cytoskeleton organization
neuron differentiation
organ regeneration
neuron projection development
actin cytoskeleton reorganization
response to insulin
cellular response to insulin stimulus
response to nicotine
intracellular signal transduction
regulation of growth
regulation of cell proliferation
response to hydrogen peroxide
positive regulation of DNA replication
positive regulation of vasoconstriction
positive regulation of smooth muscle cell proliferation
response to glucocorticoid
signal transduction
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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