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Invitrogen
Phospho-LIMK1/LIMK2 (Thr508, Thr505) Polyclonal Antibody
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FIGURE: 1 / 5
Phospho-LIMK1/LIMK2 (Thr508, Thr505) Antibody (PA5-104924) in IHC (P)
Immunohistochemistry analysis of Phospho-LIMK1/LIMK2 (Thr508, Thr505) in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-LIMK1/LIMK2 (Thr508, Thr505) polyclonal antibody (Product # PA5-104924) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody... View More
Product Details
PA5-104924
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human LIMK1(Accession P53667), corresponding to amino acid residues around phosphorylated Thr508.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of LIMK1/2 only when phosphorylated at Threonine 508/505.
Target Information
Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, including LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regulate the stabilization of F-Actin structures and cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regulation of cell motility and morphogenesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, and is thought to act by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adult testis that is thought to arise from an alternative initiation exon.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: KIZ-1; LIM domain kinase 1; LIM domain kinase 2; lim kinase 1; LIM kinase 2; LIM motif-containing protein kinase; LIM motif-containing protein kinase 1; LIM motif-containing protein kinase 2; LIM-domain containing protein kinase 1; LIM-domain containing, protein kinase 1; LIMK 2; LIMK-1; LIMK-2; LIMK2; LIMK2b; unnamed protein product
Gene Aliases: KIZ-1; LIMK; LIMK-1; Limk-2; LIMK1; LIMK2; Limk2b; Link2
UniProt ID: (Human) P53667, (Human) P53671, (Mouse) P53668, (Mouse) O54785, (Rat) P53670
Entrez Gene ID: (Human) 3984, (Human) 3985, (Mouse) 16885, (Rat) 65172, (Mouse) 16886, (Rat) 29524
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
heat shock protein binding
ubiquitin protein ligase binding
metal ion binding
protein serine kinase activity
ubiquitin ligase inhibitor activity
zinc ion binding
protein heterodimerization activity
transferase activity, transferring phosphorus-containing groups
protein phosphorylation
spermatogenesis
phosphorylation
spermatid development
signal transduction
Rho protein signal transduction
nervous system development
actin cytoskeleton organization
positive regulation of actin filament bundle assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
stress fiber assembly
positive regulation of axon extension
axon extension
positive regulation of stress fiber assembly
astral microtubule organization
establishment of vesicle localization
head development
cornea development in camera-type eye
positive regulation of protein localization to nucleus
negative regulation of cilium assembly
negative regulation of ubiquitin-protein transferase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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