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          • Primary Antibodies ›
          • IL-1 beta (Cleaved Asp116) Antibodies

          Invitrogen

          IL-1 beta (Cleaved Asp116) Polyclonal Antibody

          5 References

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          Cite IL-1 beta (Cleaved Asp116) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          IL-1 beta (Cleaved Asp116) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          IL-1 beta (Cleaved Asp116) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          IL-1 beta (Cleaved Asp116) Antibody (PA5-105048) in WB

          Western blot analysis of IL-1 beta (Cleaved Asp116) in mouse brain and mouse spleen. Samples were incubated with IL-1 beta (Cleaved Asp116) polyclonal antibody (Product # PA5-105048). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          IL-1 beta (Cleaved Asp116) Antibody in Western Blot (WB)
          IL-1 beta (Cleaved Asp116) Antibody in Western Blot (WB)

          Product Details

          PA5-105048

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (IHC)

          -
          View 3 publications 3 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat, Zebrafish

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human IL1B(Accession P01584), corresponding to amino acid residues N110-Q130.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2816521

          Product Specific Information

          Antibody detects endogenous levels of fragment of activated IL-1 beta resulting from cleavage adjacent to Asp116.

          Target Information

          IL-1 beta (Cleaved Asp116) refers to the mature, biologically active form of interleukin-1 beta generated after proteolytic processing of its inactive precursor, pro-IL-1 beta. IL-1 beta is encoded by the IL1B gene and is synthesized as a 31 kDa cytosolic precursor in response to inflammatory stimuli such as infection or tissue injury. Activation of inflammasome complexes leads to caspase-1-mediated cleavage at aspartic acid residue 116, producing the approximately 17 kDa mature cytokine. The cleaved form is released through non-classical secretion pathways and binds to the interleukin-1 receptor type 1 (IL-1R1), triggering downstream signaling through NF-kappa B and MAP kinase pathways. Mature IL-1 beta is a potent pro-inflammatory cytokine that promotes fever, leukocyte recruitment, and amplification of innate and adaptive immune responses. Detection of IL-1 beta cleaved at Asp116 is widely used as a marker of inflammasome activation and inflammatory signaling in immunology and disease research.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Catabolin; Cleaved IL-1 beta; IL-1; IL-1 beta; IL1B; interleukin 1 precursor polypeptide; interleukin 1beta; Interleukin-1 beta; interlukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta; unnamed protein product

          View more View less

          Gene Aliases: IL-1; Il-1b; IL-1beta; il1-b; IL1-BETA; IL1B; IL1beta; IL1F2; zgc:111873

          View more View less

          UniProt ID: (Human) P01584, (Mouse) P10749

          View more View less

          Entrez Gene ID: (Human) 3553, (Mouse) 16176, (Rat) 24494, (Zebrafish) 405770

          View more View less

          Function(s)
          cytokine activity interleukin-1 receptor binding integrin binding protein binding protein domain specific binding receptor agonist activity receptor binding interleukin-1 receptor antagonist activity interleukin superfamily
          Process(es)
          fever generation positive regulation of cytokine production positive regulation of T cell mediated immunity apoptotic process inflammatory response immune response signal transduction JNK cascade cell-cell signaling embryo implantation positive regulation of cell proliferation negative regulation of cell proliferation response to carbohydrate vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression positive regulation of platelet-derived growth factor receptor signaling pathway positive regulation of epithelial to mesenchymal transition positive regulation of macrophage derived foam cell differentiation negative regulation of glucose transport smooth muscle adaptation cytokine-mediated signaling pathway hyaluronan biosynthetic process positive regulation of cell migration neutrophil chemotaxis positive regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of prostaglandin biosynthetic process positive regulation of fever generation positive regulation of prostaglandin secretion response to lipopolysaccharide positive regulation of chemokine production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-2 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of immature T cell proliferation in thymus response to ATP positive regulation of heterotypic cell-cell adhesion ectopic germ cell programmed cell death positive regulation of T cell proliferation regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of MAPK cascade positive regulation of MAPK cascade positive regulation of nitric oxide biosynthetic process positive regulation of angiogenesis negative regulation of lipid metabolic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of complement activation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade negative regulation of insulin receptor signaling pathway positive regulation of protein export from nucleus astrocyte activation regulation of defense response to virus by host positive regulation of inflammatory response regulation of neurogenesis negative regulation of neurogenesis regulation of insulin secretion negative regulation of synaptic transmission defense response to Gram-positive bacterium leukocyte migration negative regulation of lipid catabolic process positive regulation of lipid catabolic process regulation of nitric-oxide synthase activity positive regulation of membrane protein ectodomain proteolysis positive regulation of cell division positive regulation of protein kinase B signaling positive regulation of glial cell proliferation positive regulation of cell adhesion molecule production negative regulation of adiponectin secretion regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade monocyte aggregation interleukin-1-mediated signaling pathway response to interleukin-1 cellular response to lipopolysaccharide cellular response to mechanical stimulus cellular response to xenobiotic stimulus positive regulation of monocyte chemotactic protein-1 production extrinsic apoptotic signaling pathway in absence of ligand cellular response to interleukin-17 positive regulation of neuroinflammatory response positive regulation of p38MAPK cascade positive regulation of NIK/NF-kappaB signaling positive regulation of RNA biosynthetic process regulation of establishment of endothelial barrier regulation of secretion by cell negative regulation of gap junction assembly positive regulation of occluding junction disassembly positive regulation of T-helper 1 cell cytokine production negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPK activity positive regulation of protein phosphorylation positive regulation of cytosolic calcium ion concentration aging memory negative regulation of gene expression positive regulation of cell death negative regulation of glutamate secretion sequestering of triglyceride lipopolysaccharide-mediated signaling pathway interleukin-1 beta production positive regulation of stress-activated MAPK cascade social behavior positive regulation of myosin light chain kinase activity cellular response to drug positive regulation of NF-kappaB import into nucleus positive regulation of apoptotic process negative regulation of MAP kinase activity protein kinase B signaling positive regulation of JUN kinase activity positive regulation of chemokine biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-6 biosynthetic process negative regulation of neuron differentiation positive regulation of glial cell differentiation positive regulation of astrocyte differentiation positive regulation of phagocytosis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of calcidiol 1-monooxygenase activity cellular response to organic substance cellular response to organic cyclic compound positive regulation of neutrophil chemotaxis negative regulation of branching morphogenesis of a nerve negative regulation of neural precursor cell proliferation response to hypoxia chronic inflammatory response to antigenic stimulus purine nucleobase metabolic process response to nutrient learning or memory estrogen metabolic process glycoprotein metabolic process response to heat response to ozone response to organonitrogen compound response to gamma radiation response to organic cyclic compound pentacyclic triterpenoid metabolic process polyketide metabolic process ovulation response to estradiol response to vitamin D response to L-ascorbic acid response to stilbenoid response to diuretic response to statin wound healing response to drug response to morphine response to peptide hormone response to ethanol response to glucocorticoid cellular response to antibiotic cellular response to glucose stimulus cellular response to lipid cellular response to fatty acid cellular response to methotrexate response to dexamethasone response to molecule of bacterial origin response to virus response to bacterium fin regeneration neutrophil activation defense response to bacterium response to exogenous dsRNA response to arsenic-containing substance macrophage chemotaxis regulation of NIK/NF-kappaB signaling negative regulation of interleukin-1-mediated signaling pathway
          It has to be done as per old AB suggested Products section.

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