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Invitrogen
Phospho-DKK1 (Ser140) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-DKK1 (Ser140) Antibody (PA5-105051) in WB
Western blot analysis of Phospho-DKK1 (Ser140) in extracts from various samples. Samples were incubated with Phospho-DKK1 (Ser140) polyclonal antibody (Product # PA5-105051). Lane 1: Mouse lung, blocked with antigen-specific peptides; Lane 2: Mouse lung; Lane 3: RAW264.7 cells (LPS 4h treatment).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105051
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DKK1(Accession O94907), corresponding to amino acid residues around phosphorylated Ser140.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of DKK1 only when phosphorylated at Ser140.
Target Information
DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head-forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play important regulatory roles in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. It has been suggested that by inhibiting Wnt/beta-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dickkopf-1; dickkopf-like protein 1; Dickkopf-related protein 1; Dickkopf1; dkk-1; hDkk1; SK
Gene Aliases: DKK1; mdkk-1; UNQ492/PRO1008
UniProt ID: (Mouse) O54908
Entrez Gene ID: (Mouse) 13380
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis involved in differentiation
endoderm formation
mesoderm formation
hair follicle development
regulation of receptor internalization
multicellular organism development
endoderm development
Wnt signaling pathway
regulation of Wnt signaling pathway
negative regulation of Wnt signaling pathway
embryonic limb morphogenesis
negative regulation of BMP signaling pathway
forebrain development
negative regulation of protein complex assembly
response to retinoic acid
negative regulation of peptidyl-serine phosphorylation
negative regulation of mesodermal cell fate specification
regulation of endodermal cell fate specification
negative regulation of skeletal muscle tissue development
head morphogenesis
face morphogenesis
negative regulation of pathway-restricted SMAD protein phosphorylation
positive regulation of heart induction by negative regulation of canonical Wnt signaling pathway
negative regulation of canonical Wnt signaling pathway
Wnt signaling pathway involved in somitogenesis
extracellular negative regulation of signal transduction
negative regulation of canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment
negative regulation of receptor activity
negative regulation of cardiac muscle cell differentiation
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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