Search
Invitrogen
Phospho-IRAK2 (Tyr6) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
Phospho-IRAK2 (Tyr6) Antibody (PA5-105120) in WB
Western blot analysis of Phospho-IRAK2 (Tyr6) in 293T cell lysate (Lane 1: treated with phospho-blocking peptide; Lane 2: treated with non-phospho-blocking peptide). Samples were incubated with Phospho-IRAK2 (Tyr6) polyclonal antibody (Product # PA5-105120).
Product Details
PA5-105120
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human IRAK2(Accession O43187), corresponding to amino acid residues around phosphorylated Tyr6.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of IRAK2 only when phosphorylated at Tyr6.
Target Information
IRAK2 (interleukin-1 receptor-associated kinase-like 2) binds to the IL-1 type I receptor following IL-1 engagment and triggers intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kappa-B, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kappa-B activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kappa-B activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory responses to cytokine IL-1 and LPS. IRAK2 is expressed in a variety of human tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: a novel IRAK/Pelle family member; interleukin-1 receptor-associated kinase 2c; Interleukin-1 receptor-associated kinase-like 2; IRAK-2; MGC150550; mu-IRAK-2; unnamed protein product
Gene Aliases: 6330415L08Rik; AI649099; IRAK-2; IRAK2
UniProt ID: (Human) O43187, (Mouse) Q8CFA1, (Rat) Q4QQS0
Entrez Gene ID: (Human) 3656, (Mouse) 108960, (Rat) 362418
nucleotide binding
protein kinase activity
protein binding
ATP binding
signaling adaptor activity
protein homodimerization activity
protein heterodimerization activity
binding, bridging
protein serine/threonine kinase activity
transferase activity, transferring phosphorus-containing groups
molecular_function
regulation of cytokine-mediated signaling pathway
toll-like receptor signaling pathway
protein phosphorylation
signal transduction
activation of NF-kappaB-inducing kinase activity
lipopolysaccharide-mediated signaling pathway
negative regulation of NF-kappaB transcription factor activity
intracellular signal transduction
regulation of apoptotic process
negative regulation of sequence-specific DNA binding transcription factor activity
protein autophosphorylation
positive regulation of NF-kappaB transcription factor activity
interleukin-1-mediated signaling pathway
response to interleukin-1
MyD88-dependent toll-like receptor signaling pathway
inflammatory response
I-kappaB kinase/NF-kappaB signaling
Toll signaling pathway
toll-like receptor 4 signaling pathway
NIK/NF-kappaB signaling
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
biological_process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support