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          • Primary Antibodies ›
          • GATA3 Antibodies

          Invitrogen

          GATA3 Polyclonal Antibody

          View all (81) GATA3 antibodies

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          Cite GATA3 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          GATA3 Antibody (PA5-105295) in ICC/IF

          Immunocytochemistry analysis of GATA3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with GATA3 polyclonal antibody (Product # PA5-105295) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          GATA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GATA3 Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          GATA3 Polyclonal Antibody

          Product Details

          PA5-105295

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human GATA3(Accession P23771), corresponding to amino acid residues C284-G334.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2853888

          Product Specific Information

          Antibody detects endogenous levels of total GATA3.

          Target Information

          The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: GATA binding protein-3; GATA-3; GATA-binding factor 3; GATA-binding protein 3; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3; unnamed protein product

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          Gene Aliases: Gata-3; GATA3; HDR; HDRS; jal

          View more View less

          UniProt ID: (Mouse) P23772

          View more View less

          Entrez Gene ID: (Human) 2625, (Mouse) 14462, (Rat) 85471

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          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcription coactivator binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding interleukin-2 receptor binding protein binding zinc ion binding identical protein binding sequence-specific DNA binding RNA polymerase II sequence-specific DNA binding transcription factor binding E-box binding HMG box domain binding histone methyltransferase binding sequence-specific double-stranded DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter sequence-specific DNA binding nucleic acid binding transcription factor activity transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding enhancer sequence-specific DNA binding transcription coactivator activity transcription factor binding transcription regulatory region DNA binding metal ion binding protein dimerization activity DNA-binding transcription factor
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter cell fate determination type IV hypersensitivity regulation of cytokine production kidney development mesonephros development immune system development aortic valve morphogenesis cardiac right ventricle morphogenesis ventricular septum development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter defense response inflammatory response signal transduction negative regulation of cell proliferation male gonad development response to xenobiotic stimulus response to virus anatomical structure morphogenesis positive regulation of signal transduction response to gamma radiation gene expression positive regulation of endothelial cell migration negative regulation of epithelial to mesenchymal transition cell differentiation T cell differentiation macrophage differentiation regulation of epithelial cell differentiation TOR signaling positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of interleukin-5 production negative regulation of mammary gland epithelial cell proliferation cellular response to interferon-alpha norepinephrine biosynthetic process protein kinase B signaling ear development response to estrogen T-helper 2 cell differentiation cell fate commitment response to ethanol positive regulation of T cell differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter sympathetic nervous system development embryonic organ development anatomical structure formation involved in morphogenesis system development negative regulation of inflammatory response regulation of developmental process T cell receptor signaling pathway cartilage development positive regulation of protein kinase B signaling pharyngeal system development uterus development canonical Wnt signaling pathway mesenchymal to epithelial transition ureteric bud formation cellular response to interleukin-4 cellular response to tumor necrosis factor positive regulation of ureteric bud formation nephric duct morphogenesis nephric duct formation regulation of nephron tubule epithelial cell differentiation lymphocyte migration cochlea development positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of cell motility negative regulation of endothelial cell apoptotic process positive regulation of thyroid hormone generation positive regulation of transcription regulatory region DNA binding regulation of cellular response to X-ray cell morphogenesis in utero embryonic development neuron migration cell activation positive regulation of cytokine production lens development in camera-type eye immune system process pro-T cell differentiation transcription, DNA-templated transcription from RNA polymerase II promoter humoral immune response nervous system development axon guidance post-embryonic development negative regulation of gene expression regulation of neuron projection development phosphatidylinositol 3-kinase signaling neuron differentiation erythrocyte differentiation negative regulation of interferon-gamma production negative regulation of interleukin-2 production T cell differentiation in thymus embryonic hemopoiesis ureter maturation parathyroid hormone secretion regulation of cytokine biosynthetic process inner ear morphogenesis regulation of CD4-positive, alpha-beta T cell differentiation regulation of neuron apoptotic process thymic T cell selection innate immune response positive regulation of cell differentiation negative regulation of fat cell differentiation cell maturation thymus development developmental growth homeostasis of number of cells regulation of histone H3-K4 methylation parathyroid gland development mast cell differentiation regulation of histone H3-K27 methylation canonical Wnt signaling pathway involved in metanephric kidney development cellular response to cytokine stimulus otic vesicle development cellular response to BMP stimulus renal system development interleukin-4 secretion interferon-gamma secretion regulation of establishment of cell polarity negative regulation of cell proliferation involved in mesonephros development positive regulation of interleukin-5 secretion positive regulation of interleukin-13 secretion negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation response to drug
          It has to be done as per old AB suggested Products section.

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